The protocol we present here will be helpful for us to understand how lymphocytes are involved in the development of the central nervous system autoimmune disease. With this method, lymphocytes in the brain can be studied studied on a cell by cell
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This manuscript presents a protocol to induce active experimental autoimmune encephalomyelitis (EAE) in mice. A method for the isolation and characterization of the infiltrated lymphocytes in the central nervous system (CNS) is also presented to show how lymphocytes are involved in the development of CNS autoimmune disease.