Phase II: Preparation of the Alcohol Insoluble Crude Cell Wall
4:52
Phase III: Removal of Hemicellulose and Lignin using Updegraff Reagent
7:10
Phase IV: Acid Hydrolysis of Cellulose into Glucose Monomers
7:49
Phase V: Anthrone Assay to Estimate the Glucose Concentration of Samples and Glucose Standard
10:04
Representative Results & Conclusion
Transcripción
Here, we demonstrate Updegraff method, the world's most widely used method for the estimation of crystalline cellulose content. Cellulose is basically made up of glucose monomers linked by beta-1, 4 glycosidic bonds. We divided this protocol into
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The Updegraff method is the most widely used method for the cellulose estimation. The main purpose of this demonstration is to provide a detailed Updegraff protocol for estimation of cellulose content in plant biomass samples.