May 16th, 2021
•Monitoring changes in the melting temperature of a target protein (i.e., thermal shift assay, TSA) is an efficient method for screening fragment libraries of a few hundred compounds. We present a TSA protocol implementing robotics-assisted nano-Differential Scanning Fluorimetry (nano-DSF) for monitoring intrinsic tryptophan fluorescence and light back-scattering for fragment screening.
Videos relacionados
Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography
Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase
Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms
Microcrystallography of Protein Crystals and In Cellulo Diffraction
A Simple Method for High Throughput Chemical Screening in Caenorhabditis Elegans
Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain
Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions
How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project
Kinase Inhibitor Screening In Self-assembled Human Protein Microarrays
Monitoring GPCR-β-arrestin1/2 Interactions in Real Time Living Systems to Accelerate Drug Discovery
ACERCA DE JoVE
Copyright © 2024 MyJoVE Corporation. Todos los derechos reservados