<meta charset="utf8"></head><body>Nanocompuestos antibacterianos de óxido de grafeno/cobre para bacterias resistentes

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A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Sonochemical+synthesis+of+a+copper+reduced+graphene+oxide+nanocomposite+using+honey+and+evaluation+of+its+antibacterial+and+cytotoxic+activities.">Sonochemical synthesis of a copper reduced graphene oxide nanocomposite using honey and evaluation of its antibacterial and cytotoxic activities.</a> Front Mol Biosci. 9, 995853 (2022).</li><li>Tu, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Remarkable+antibacterial+activity+of+reduced+graphene+oxide+functionalized+by+copper+ions.">Remarkable antibacterial activity of reduced graphene oxide functionalized by copper ions.</a> Adv Funct Mater. 31 (13), 2008018 (2021).</li><li>Li, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Toward+a+molecular+understanding+of+the+antibacterial+mechanism+of+copper-bearing+titanium+alloys+against+staphylococcus+aureus.">Toward a molecular understanding of the antibacterial mechanism of copper-bearing titanium alloys against staphylococcus aureus.</a> Adv Healthc Mater. 5 (5), 557-566 (2016).</li><li>Yang, Z., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Long-term+antibacterial+stable+reduced+graphene+oxide+nanocomposites+loaded+with+cuprous+oxide+nanoparticles.">Long-term antibacterial stable reduced graphene oxide nanocomposites loaded with cuprous oxide nanoparticles.</a> J Colloid Interface Sci. 533, 13-23 (2019).</li><li>Yang, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Graphene+oxide/copper+nanoderivatives-modified+chitosan/hyaluronic+acid+dressings+for+facilitating+wound+healing+in+infected+full-thickness+skin+defects.">Graphene oxide/copper nanoderivatives-modified chitosan/hyaluronic acid dressings for facilitating wound healing in infected full-thickness skin defects.</a> Int J Nanomedicine. 15, 8231-8247 (2020).</li><li>Mao, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Graphene+oxide-copper+nanocomposites+suppress+cariogenic+streptococcus+mutans+biofilm+formation.">Graphene oxide-copper nanocomposites suppress cariogenic streptococcus mutans biofilm formation.</a> Int J Nanomedicine. 16, 7727-7739 (2021).</li><li>Ravikumar, V., Mijakovic, I., Pandit, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Antimicrobial+activity+of+graphene+oxide+contributes+to+alteration+of+key+stress-related+and+membrane+bound+proteins.">Antimicrobial activity of graphene oxide contributes to alteration of key stress-related and membrane bound proteins.</a> Int J Nanomedicine. 17, 6707-6721 (2022).</li><li>Afreen, G., Shoeb, M., Upadhyayula, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effectiveness+of+reactive+oxygen+species+generated+from+rgo/cds+qd+heterostructure+for+photodegradation+and+disinfection+of+pollutants+in+waste+water.">Effectiveness of reactive oxygen species generated from rgo/cds qd heterostructure for photodegradation and disinfection of pollutants in waste water.</a> Mater Sci Eng C. 108, 110372 (2020).</li><li>Yang, F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fabrication+of+graphene+oxide/copper+synergistic+antibacterial+coating+for+medical+titanium+substrate.">Fabrication of graphene oxide/copper synergistic antibacterial coating for medical titanium substrate.</a> J Colloid Interface Sci. 638, 1-13 (2023).</li><li>Rajapaksha, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Antibacterial+properties+of+graphene+oxide-copper+oxide+nanoparticle+nanocomposites.">Antibacterial properties of graphene oxide-copper oxide nanoparticle nanocomposites.</a> ACS Appl Bio Mater. 2 (12), 5687-5696 (2019).</li><li>Sachdeva, C., Satyamoorthy, K., Murali, T. 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M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+role+for+the+gram-negative+outer+membrane+in+bacterial+shape+determination.">A role for the gram-negative outer membrane in bacterial shape determination.</a> PNAS. 120 (35), e2301987120 (2023).</li><li>Wu, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Copper-containing+mesoporous+bioactive+glass+scaffolds+with+multifunctional+properties+of+angiogenesis+capacity,+osteostimulation+and+antibacterial+activity.">Copper-containing mesoporous bioactive glass scaffolds with multifunctional properties of angiogenesis capacity, osteostimulation and antibacterial activity.</a> Biomater. 34 (2), 422-433 (2013).</li><li>Zhang, W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Graphene+oxide-copper+nanocomposite-coated+porous+cap+scaffold+for+vascularized+bone+regeneration+via+activation+of+hif-1&#945;.">Graphene oxide-copper nanocomposite-coated porous cap scaffold for vascularized bone regeneration via activation of hif-1&#945;.</a> Adv Healthc Mater. 5 (11), 1299-1309 (2016).</li><li>Labauve, A. 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<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>Antibiotic-Antimycotic</td>
			<td>Gibco</td>
			<td>15240062</td>
			<td></td>
		</tr>
		<tr>
			<td>Clinical MDR bacterial strains&#160;</td>
			<td>Chung-Ang University Hospital (Seoul, South Korea)</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Copper(II) chloride dihydrate</td>
			<td>Duksan</td>
			<td>10125-13-0</td>
			<td></td>
		</tr>
		<tr>
			<td>Field Emission Scanning Electron Microscope (FE-SEM)</td>
			<td>Carl Zeiss</td>
			<td>SIGMA</td>
			<td></td>
		</tr>
		<tr>
			<td>Graphene oxide</td>
			<td>Sigma</td>
			<td>796034</td>
			<td></td>
		</tr>
		<tr>
			<td>Sodium Borohydride</td>
			<td>Sigma</td>
			<td>71320</td>
			<td></td>
		</tr>
		<tr>
			<td>Transmission Electron Microscopy (TEM)</td>
			<td>JEOL</td>
			<td>JEM-2100</td>
			<td></td>
		</tr>
		<tr>
			<td>Tryptic Soy Agar</td>
			<td>BD difco</td>
			<td>236950</td>
			<td></td>
		</tr>
		<tr>
			<td>Tryptic Soy Broth</td>
			<td>BD difco</td>
			<td>211825</td>
			<td></td>
		</tr>
	</tbody>
</table>

fabrication of antibacterial graphene oxide/copper nanocomposites

Bacterial infections have a significant impact on public health. Pathogenic bacteria, in particular, can evade the body&#39;s protective mechanisms and cause diseases1. Antibiotics are widely used to treat bacterial infections. However, the inappropriate use of antibiotics has precipitated the emergence of antibiotic-resistant bacteria. Currently, nosocomial infections attributable to antibiotic-resistant bacteria have caused notable complications in healthcare facilities2. Unfortunately, bacteria have resistance mechanisms for all current antibiotics3. Therefore, developing new antibiotics is essential,&#160;though&#160;there is also a high probability that resistance mechanisms will emerge.
Metal nanoparticles have emerged as promising agents for combating antibiotic-resistant bacteria because of their effective antibacterial properties4,5,6. It is difficult for bacteria to develop resistance mechanisms against metal nanoparticles because they do not bind to any specific bacterial receptors7. In particular, silver, copper, and zinc oxide nanoparticles are the focus of extensive research because of their remarkable antibacterial efficacy8,9,10,11,12,13. The toxicity of metal nanoparticles toward bacteria is attributed to three primary mechanisms: 1) the release of metal ions, 2) the generation of reactive oxygen species (ROS), such as &#8226;OH and &#8226;O2&#8722;, and 3) physical interaction and attachment4,14.
In this study, graphene oxide/copper (GO/Cu) nanocomposites were developed as an antimicrobial agent. Copper (Cu) efficiently kills pathogens that encounter its surface and interferes with bacterial gene replication. However, it is an essential microelement in the human body, and it is less toxic to mammalian cells because it has homeostatic mechanisms that regulate the concentrations of Cu inside the cells4. When Cu nanoparticles are oxidized, they generate Cu ions, which have a relatively high affinity for negatively charged bacteria15,16. Cu ions bind to the cellular structures (e.g., proteins, membranes, and DNA), disrupting cell functions17. By synthesizing Cu nanoparticles on the surface of graphene oxide (GO), the release rate of metal ions can be controlled18,19,20. GO also shows an antibacterial effect by preventing bacterial adhesion with a rough surface through oxidative stress or by forming ROS21,22. Moreover, GO/Cu nanocomposites release more ROS than GO through the chemical action of Cu2+, which damages bacterial proteins and DNA, leading to the death of bacteria23,24.
This paper outlines the protocol for synthesizing GO/Cu nanocomposites and introduces an antimicrobial testing approach against clinical bacteria MRSA and P. aeruginosa, the most common multidrug-resistant bacteria strains that cause nosocomial infections25. This protocol aims to introduce a facile chemical reduction method for synthesizing GO/Cu nanocomposites that can impede the growth of antibiotic-resistant bacteria. We use copper(II) chloride (CuCl2) and sodium borohydride (NaBH4) as a precursor and reducing agent, respectively. The protocol also describes the specifics of applying these nanocomposites to bacteria using the microdilution broth method. To exclude errors due to the absorption interference of metal nanoparticles and make accurate evaluations, the antibacterial ability is evaluated using the colony counting method.

<meta charset="utf8"></head><body>Autor destacado: Nanocompuestos metálicos para eliminar bacterias resistentes a los antibióticos

Fabricación de nanocompuestos antibacterianos de óxido de grafeno/cobre

Antibiotics are widely used to treat bacterial infections, but overusing them has led to bacteria becoming resistant. To address this problem we've been working on developing anti bacteria metallic nanocomposites which make a tougher bacteria to develop resistance mechanisms. The advantage of this protocol is that it is a low cost and easy method for making geo copper nanocomposites compared to other protocols.This makes it practical for large scale production which is great for commercial use. Also, our method doesn't need extra disinfectants or capping agents making it an option for biomedical applications. Our group is focused on the developing bio comparable metallic nanocomposites for infection treatment.Therefore we are working on coding these nanocomposites onto surfaces or using them to deliver drugs aiming to effectively eradicate antibody resistant bacteria and their biofilms in real world infection scenarios.

Antibiotics are currently the most used antibacterial treatment for killing bacteria. However, bacteria develop resistance when continually overexposed to ...

fabrication-of-antibacterial-graphene-oxidecopper-nanocomposites

Without Section

Research

Fabrication of Antibacterial Graphene Oxide/Copper Nanocomposites

JoVE Journal

Bioengineering

416 vistas.  Chung-Ang University. Los antibióticos se usan ampliamente para tratar infecciones bacterianas, pero su uso excesivo ha llevado a que las bacterias se vuelvan resistentes. Para abordar este problema, hemos estado trabajando en el desarrollo de nanocompuestos metálicos antibacterianos, que hacen que las bacterias sean más resistentes a desarrollar mecanismos de resistencia. La ventaja de este protocolo es que es un método fácil y de bajo costo para hacer nanocompuestos de geoc...