methyl-binding-dna-capture-sequencing-for-patient-tissues

Methyl-binding DNA capture Sequencing for Patient Tissues

Methylation is one of the essential epigenetic modifications to the DNA, which is responsible for the precise regulation of genes required for stable ...

genome-wide-analysis-hdac-inhibitor-mediated-modulation-micrornas

Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation

Antibody responses are accomplished through several critical B cell-intrinsic processes, including somatic hypermutation (SHM), class-switch DNA ...

University of Texas Health Science Center at San Antonio

University of Texas Long School of Medicine at San Antonio

Grace under fire.

The loss of the Y chromosome is a frequent numerical chromosomal abnormality observed in human prostate cancer. In cancer, loss of specific genetic material frequently accompanies simultaneous inactivation of tumor suppressor genes. It is not known whether the Y chromosome harbors such genes. To address the role of genes on the Y chromosome in human prostate cancer, we transferred a tagged Y chromosome into PC-3, a human prostate cancer cell line lacking a Y chromosome. A human Y chromosome was tagged with the hisD gene and transferred to PC-3 by microcell-mediated chromosome transfer. Tumorigenicity of these PC-3 hybrids was tested in vivo and in vitro, and the results were compared with those of the polymerase chain reaction analyses conducted on the PC-3 hybrids using Y chromosome-specific markers. Among 60 mice injected with 12 different PC-3 hybrids (five mice per hybrid), tumor growth was apparent in only one mouse, whereas tumors grew in all mice injected with the parental PC-3 cells. An in vitro assay showed that the Y chromosome did not suppress anchorage-independent growth of PC-3 cells. We found that addition of the Y chromosome suppressed tumor formation by PC-3 in athymic nude mice, and that this block of tumorigenesis was independent of the in vitro growth properties of the cells. This observation suggests the presence of a gene important for prostate tumorigenesis on the Y chromosome.

The human Y chromosome suppresses the tumorigenicity of PC-3, a human prostate cancer cell line, in athymic nude mice.

Prostate cancer is the second leading cause of cancer deaths among American men. The loss of Y chromosome has been frequently observed in primary prostate cancer as well as other types of cancer. Earlier, we showed that introduction of the human Y chromosome suppresses the in vivo tumorigenicity of the prostate cancer cell line PC-3. To further characterize the Y chromosome, we have developed a high-density bacterial artificial chromosome (BAC) microarray containing 178 BAC clones from the human Y chromosome. BAC microarray was used for array comparative genomic hybridization on prostate cancer samples and cell lines. The most prominent observation on prostate cancer specimens was a deletion at Yp11.2 containing the TSPY tandem gene array. Out of 36 primary prostate tumors analyzed, 16 (44.4%) samples exhibited loss of TSPY gene copies. Notably, we observed association between the number of TSPY copies in the blood and the incidence of prostate cancer. Moreover, PC-3 hybrids with an intact Yp11.2 did not grow tumors in nude mice, whereas PC-3 hybrids with a deletion at Yp11.2 grew tumors in nude mice.

Detection of recurrent copy number loss at Yp11.2 involving TSPY gene cluster in prostate cancer using array-based comparative genomic hybridization.

After the completion of a draft human genome sequence, the International Human Genome Sequencing Consortium has proceeded to finish and annotate each of the 24 chromosomes comprising the human genome. Here we describe the sequencing and analysis of human chromosome 3, one of the largest human chromosomes. Chromosome 3 comprises just four contigs, one of which currently represents the longest unbroken stretch of finished DNA sequence known so far. The chromosome is remarkable in having the lowest rate of segmental duplication in the genome. It also includes a chemokine receptor gene cluster as well as numerous loci involved in multiple human cancers such as the gene encoding FHIT, which contains the most common constitutive fragile site in the genome, FRA3B. Using genomic sequence from chimpanzee and rhesus macaque, we were able to characterize the breakpoints defining a large pericentric inversion that occurred some time after the split of Homininae from Ponginae, and propose an evolutionary history of the inversion.

The DNA sequence, annotation and analysis of human chromosome 3.

SEMA3B and SEMA3F are 2 closely related genes lying 80 kb apart on chromosome 3 that have been shown to suppress tumor formation in vivo and in vitro. Each gene has a single nucleotide polymorphism that results in a nonsynonymous coding change, rs2071203 (SEMA3B) and rs1046956 (SEMA3F), as well as noncoding single nucleotide polymorphisms.

Semaphorin 3B and 3F single nucleotide polymorphisms are associated with prostate cancer risk and poor prognosis.

Since the beginning of the Overseas Contingency Operation, more than 45,000 ill and wounded service members have been evacuated from the battlefield to Landstuhl Regional Medical Center (LRMC) in western Europe. LRMC is a stopover for these service members, where they are further assessed, treated, and stabilized before they return to the United States. This process requires coordination between different military services, health care teams, and modes of transportation. These processes can be complicated given the severity of the wounded. Nurses at LRMC have learned how to streamline services, providing efficient, comprehensive care for wounded service members and their families.

Rewards and challenges of nursing wounded warriors at Landstuhl Regional Medical Center, Germany.

&#x5DE5;&#x4F5C;&#x8D1F;&#x8377;&#x7BA1;&#x7406;&#x7CFB;&#x7EDF;&#x7684;&#x62A4;&#x7406; &#xFF08;WMSN&#xFF09; &#x662F;&#x5DF2;&#x63D0;&#x4F9B;&#x8FD1;&#x5B9E;&#x65F6;&#x3001; &#x5168;&#x9762;&#x62A4;&#x7406;&#x5DE5;&#x4F5C;&#x91CF;&#x548C;&#x4EBA;&#x529B;&#x6570;&#x636E;&#x51B3;&#x7B56;&#x5236;&#x5B9A;&#x5404;&#x7EA7;&#x8D85;&#x8FC7; 25 &#x5E74;&#x7684;&#x9646;&#x519B;&#x519B;&#x533B;&#x90E8;&#x4E1A;&#x52A1;&#x7CFB;&#x7EDF;&#x7684;&#x6838;&#x5FC3;&#x3002;&#x519B;&#x961F;&#x7684;&#x4EBA;&#x624B;&#x9700;&#x6C42;&#x548C;&#x6587;&#x4EF6;&#x5904;&#x586B;&#x5145; WMSN &#x7684;&#x6570;&#x636E;&#x7EB3;&#x5165;&#x7EDF;&#x7B79;&#x4EBA;&#x5458;&#x914D;&#x7F6E;&#x6807;&#x51C6;&#x7CFB;&#x7EDF; &#xFF08;&#x81EA;&#x52A8;&#x7F16;&#x5236;&#x8BC4;&#x4F30;&#x6A21;&#x578B;&#x5185;&#x4F4F;&#x9662;&#x6A21;&#x5757;&#xFF09;&#x3002;&#x76EE;&#x524D;&#x7684;&#x5236;&#x5EA6;&#xFF0C;&#x5DE5;&#x4F5C;&#x8D1F;&#x8377;&#x7BA1;&#x7406;&#x7CFB;&#x7EDF;&#x7684;&#x62A4;&#x7406;&#x4E92;&#x8054;&#x7F51; &#xFF08;WMSNi&#xFF09;&#xFF0C;&#x662F;&#x73B0;&#x4EE3;&#x5316;&#x548C;&#x4E00;&#x4F53;&#x5316;&#x4F01;&#x4E1A;&#x4E00;&#x7EA7;&#x9700;&#x8981;&#x989D;&#x5916;&#x529F;&#x80FD;&#x7684;&#x4E34;&#x65F6;&#x89E3;&#x51B3;&#x65B9;&#x6848;&#x3002;&#x6269;&#x5C55;&#x7684;&#x7279;&#x6D3E;&#x56E2;&#x548C;&#x6279;&#x51C6;&#x8981;&#x6C42;&#x7684;&#x5173;&#x4E8E;&#x519B;&#x961F;&#x533B;&#x7597;&#x547D;&#x4EE4; WMSNi &#x652F;&#x6301;&#x6218;&#x7565;&#x5021;&#x8BAE;&#x5E73;&#x8861;&#x8BB0;&#x5206;&#x5361;&#x548C;&#x591A;&#x4E2A;&#x4EBA;&#x5458;&#x7EDF;&#x7B79;&#x4E1A;&#x52A1;&#x6D41;&#x7A0B;&#xFF0C;&#x4E3A;&#x4F4F;&#x9662;&#x75C5;&#x4EBA;&#x548C;&#x95E8;&#x8BCA;&#x75C5;&#x4EBA;&#x7684;&#x62A4;&#x7406;&#x9700;&#x8981;&#x7EE7;&#x7EED;&#x7684;&#x7EF4;&#x6301;&#x3002;&#x6B64;&#x7CFB;&#x7EDF;&#x76EE;&#x524D;&#x7531; TRICARE &#x7BA1;&#x7406;&#x6D3B;&#x52A8;&#x4F5C;&#x4E3A;&#x4E34;&#x65F6;&#x591A;&#x4E1A;&#x52A1;&#x89E3;&#x51B3;&#x65B9;&#x6848;&#xFF0C;&#x5145;&#x5206;&#x5229;&#x7528;&#xFF0C;&#x5E76;&#x4E14;&#x6B63;&#x5728;&#x4F7F;&#x7528; 24 &#x519B;&#x961F;&#x533B;&#x7597;&#x8BBE;&#x65BD;&#x3002;&#x901A;&#x8FC7;&#x5728; WMSNi &#x4E2D;&#x4F7F;&#x7528;&#x7684;&#x65B9;&#x6CD5;&#x5411;&#x519B;&#x961F;&#x51B3;&#x7B56;&#x8005;&#x63D0;&#x4F9B;&#x7684;&#x57FA;&#x4E8E;&#x8BC1;&#x636E;&#x8D44;&#x6599;&#x5FC5;&#x987B;&#x8DE8;&#x519B;&#x961F;&#x533B;&#x7597;&#x547D;&#x4EE4;&#x5728;&#x6574;&#x4E2A;&#x7CFB;&#x7EDF;&#x7684;&#x751F;&#x547D;&#x5468;&#x671F;&#x3002;

&#x8861;&#x91CF;&#x62A4;&#x7406;&#x8D28;&#x91CF;&#x62A4;&#x7406;&#x75C5;&#x4EBA;&#x7ED3;&#x679C;&#x6781;&#x4E3A;&#x91CD;&#x8981;&#x7684;&#x8D21;&#x732E;&#x3002;

Workload Management System for Nursing (WMSN) is a core Army Medical Department business system that has provided near real-time, comprehensive nursing workload and manpower data for decision making at all levels for over 25 years. The Army Manpower Requirements and Documentation Agency populates data from WMSN into the Manpower Staffing Standards System (Inpatient module within Automated Staffing Assessment Model). The current system, Workload Management System for Nursing Internet (WMSNi), is an interim solution that requires additional functionalities for modernization and integration at the enterprise level. The expanding missions and approved requirements for WMSNi support strategic initiatives on the Army Medical Command balanced scorecard and require continued sustainment for multiple personnel and manpower business processes for both inpatient and outpatient nursing care. This system is currently being leveraged by the TRICARE Management Activity as an interim multiservice solution, and is being used at 24 Army medical treatment facilities. The evidenced-based information provided to Army decision makers through the methods used in the WMSNi will be essential across the Army Medical Command throughout the system's life cycle.

Measuring nursing essential contributions to quality patient care outcomes.

Syst&egrave;me de gestion de la charge de travail pour les soins infirmiers (WMSN) est un noyau de syst&egrave;me d&#39;entreprise de service de l&#39;arm&eacute;e qui a fourni pr&egrave;s de globale de donn&eacute;es de charge de travail et la main-d&#39;&oelig;uvre pour prendre des d&eacute;cisions de soins infirmiers, en temps r&eacute;el &agrave; tous les niveaux depuis plus de 25 ans. L&#39;arm&eacute;e Manpower exigences et Documentation Agence remplit les donn&eacute;es de WMSN dans le syst&egrave;me de normes de dotation Manpower (module de patients hospitalis&eacute;s au sein du mod&egrave;le d&#39;&eacute;valuation automatis&eacute;e et dotation). Le syst&egrave;me actuel, le Workload Management System for Nursing Internet (WMSNi), est une solution provisoire qui requiert des fonctionnalit&eacute;s suppl&eacute;mentaires pour la modernisation et l&#39;int&eacute;gration au niveau de l&#39;entreprise. Les missions en expansion et a approuv&eacute; les exigences de WMSNi appuyer les initiatives strat&eacute;giques sur le commandement m&eacute;dical de l&#39;arm&eacute;e balanced scorecard et n&eacute;cessitent le soutien continu pour plusieurs processus de gestion du personnel et de main-d&#39;oeuvre pour les patients hospitalis&eacute;s et ambulatoires, soins infirmiers. Ce syst&egrave;me est mobilis&eacute; par l&#39;activit&eacute; de gestion TRICARE comme solution provisoire multiservice et est utilis&eacute; dans les installations de traitement m&eacute;dical 24 arm&eacute;e. Les informations fond&eacute;es sur fournies aux d&eacute;cideurs d&#39;arm&eacute;e &agrave; travers les m&eacute;thodes utilis&eacute;es dans le WMSNi sera essentielles &agrave; travers le commandement m&eacute;dical de l&#39;arm&eacute;e tout au long du cycle de vie du syst&egrave;me.

Mesure des contributions essentielles aux r&eacute;sultats de qualit&eacute; des soins aux patients de soins infirmiers.

Workload-Management-System f&uuml;r Krankenpflege (WMSN) ist ein Kern Army Medical Department-Business-System, das in Echtzeit, umfassende Krankenpflege Arbeitsauslastung und Manpower Daten zur Entscheidungsfindung in der N&auml;he aller seit &uuml;ber 25 Jahren Ebenen hat. Die Armee Personalbedarf und Dokumentation Agentur f&uuml;llt Daten aus WMSN in die Manpower Personal Normensystem (station&auml;re Modul innerhalb Staffing Bewertungsmodell automatisiert). Das aktuelle System, Workload-Management-System f&uuml;r Krankenpflege Internet (WMSNi), ist eine &Uuml;bergangsl&ouml;sung, die zus&auml;tzliche Funktionalit&auml;ten f&uuml;r Modernisierung und Integration auf Unternehmensebene erforderlich. Die wachsenden Aufgaben und Anforderungen zugelassen, f&uuml;r WMSNi Unterst&uuml;tzung strategische Initiativen auf der Army Medical Command Scorecard Balanced und erfordern weitere Durchhaltef&auml;higkeit f&uuml;r mehrere Mitarbeiter und Manpower Gesch&auml;ftsprozesse f&uuml;r station&auml;re und ambulante Pflege. Dieses System wird derzeit von der TRICARE Management Aktivit&auml;t als Zwischenl&ouml;sung multiservice genutzt wird, und bei 24 Armee-medizinische Behandlung-Einrichtungen verwendet wird. Belegt-basierten Angaben an Entscheidungstr&auml;ger der Armee durch die Methoden der WMSNi werden in der Army Medical Command w&auml;hrend des gesamten Lebenszyklus des Systems notwendig sein.

Messung der Krankenpflege wesentliche Beitr&auml;ge zur Qualit&auml;t Patientenversorgung Ergebnisse.

Sistema di gestione del carico di lavoro per infermieri (WMSN) &egrave; un nucleo di sistema aziendale reparto medico dell&#39;esercito che ha fornito vicino in tempo reale, globale infermieristica dati del carico di lavoro e della manodopera per il processo decisionale a tutti i livelli per oltre 25 anni. L&#39;esercito Manpower requisiti e documentazione agenzia compila dati da WMSN in Manpower personale standard di sistema (modulo di degenza all&#39;interno automatizzato di modello di valutazione personale). L&#39;attuale sistema, sistema di gestione del carico di lavoro per infermieri su Internet (WMSNi), &egrave; una soluzione provvisoria che richiede funzionalit&agrave; aggiuntive per la modernizzazione e l&#39;integrazione a livello aziendale. Le missioni d&#39;espansione e approvato requisiti per WMSNi supporto iniziative strategiche su ordine medico dell&#39;esercito balanced scorecard e richiedono costante sostegno per processi aziendali pi&ugrave; personale e delle risorse umane sia per degenza e ambulatoriali, assistenza infermieristica. Questo sistema &egrave; attualmente essere sfruttato dall&#39;attivit&agrave; TRICARE Management come soluzione provvisoria multiservizio ed &egrave; utilizzato a 24 impianti di trattamento medico dell&#39;esercito. Evidenziato-base di informazioni fornite ai decisori esercito attraverso metodi utilizzati per la WMSNi sar&agrave; essenziale attraverso l&#39;ordine medico dell&#39;esercito durante tutto il ciclo di vita del sistema.

Misurazione infermieristica contributi essenziali a risultati di qualit&agrave; cura del paziente.

&#x30EF;&#x30FC;&#x30AF;&#x30ED;&#x30FC;&#x30C9;&#x7BA1;&#x7406;&#x30B7;&#x30B9;&#x30C6;&#x30E0;&#x306E;&#x770B;&#x8B77; (WMSN) &#x30B3;&#x30A2;&#x8FD1;&#x304F;&#x30EA;&#x30A2;&#x30EB;&#x30BF;&#x30A4;&#x30E0;&#x3001;&#x5305;&#x62EC;&#x7684;&#x306A;&#x610F;&#x601D;&#x6C7A;&#x5B9A;&#x306E;&#x30EF;&#x30FC;&#x30AF;&#x30ED;&#x30FC;&#x30C9;&#x3068;&#x30DE;&#x30F3;&#x30D1;&#x30EF;&#x30FC; &#x30C7;&#x30FC;&#x30BF;&#x770B;&#x8B77;&#x3059;&#x3079;&#x3066;&#x306E; 25 &#x5E74;&#x4EE5;&#x4E0A;&#x306E;&#x30EC;&#x30D9;&#x30EB;&#x3067;&#x63D0;&#x4F9B;&#x3057;&#x3066;&#x3044;&#x308B;&#x9678;&#x8ECD;&#x533B;&#x7642;&#x90E8;&#x30D3;&#x30B8;&#x30CD;&#x30B9; &#x30B7;&#x30B9;&#x30C6;&#x30E0;&#x3067;&#x3059;&#x3002;&#x9678;&#x8ECD;&#x4EBA;&#x6750;&#x8981;&#x4EF6;&#x304A;&#x3088;&#x3073;&#x30C9;&#x30AD;&#x30E5;&#x30E1;&#x30F3;&#x30C8;&#x4EE3;&#x7406;&#x5E97;&#x3001;&#x30DE;&#x30F3;&#x30D1;&#x30EF;&#x30FC;&#x4EBA;&#x6750;&#x6D3E;&#x9063;&#x6A19;&#x6E96;&#x30B7;&#x30B9;&#x30C6;&#x30E0; (&#x5165;&#x9662;&#x30E2;&#x30B8;&#x30E5;&#x30FC;&#x30EB;&#x4EBA;&#x6750;&#x6D3E;&#x9063;&#x306E;&#x8A55;&#x4FA1;&#x30E2;&#x30C7;&#x30EB;&#x306E;&#x81EA;&#x52D5;&#x5185;) &#x306B; WMSN &#x304B;&#x3089;&#x306E;&#x30C7;&#x30FC;&#x30BF;&#x3092;&#x8A2D;&#x5B9A;&#x3057;&#x307E;&#x3059;&#x3002;&#x73FE;&#x5728;&#x306E;&#x30B7;&#x30B9;&#x30C6;&#x30E0; &#x30EF;&#x30FC;&#x30AF;&#x30ED;&#x30FC;&#x30C9;&#x7BA1;&#x7406;&#x30B7;&#x30B9;&#x30C6;&#x30E0;&#x306E;&#x770B;&#x8B77;&#x30A4;&#x30F3;&#x30BF;&#x30FC;&#x30CD;&#x30C3;&#x30C8; (WMSNi) &#x306F;&#x3001;&#x8FD1;&#x4EE3;&#x5316;&#x3068;&#x30A8;&#x30F3;&#x30BF;&#x30FC;&#x30D7;&#x30E9;&#x30A4;&#x30BA; &#x30EC;&#x30D9;&#x30EB;&#x3067;&#x306E;&#x7D71;&#x5408;&#x306E;&#x305F;&#x3081;&#x306E;&#x8FFD;&#x52A0;&#x6A5F;&#x80FD;&#x304C;&#x5FC5;&#x8981;&#x3067;&#x3059;&#x3001;&#x4E2D;&#x9593;&#x306E;&#x30BD;&#x30EA;&#x30E5;&#x30FC;&#x30B7;&#x30E7;&#x30F3;&#x3067;&#x3059;&#x3002;&#x62E1;&#x5927;&#x306E;&#x30DF;&#x30C3;&#x30B7;&#x30E7;&#x30F3;&#x3068; WMSNi &#x30B5;&#x30DD;&#x30FC;&#x30C8;&#x6226;&#x7565;&#x7684;&#x30A4;&#x30CB;&#x30B7;&#x30A2;&#x30C6;&#x30A3;&#x30D6;&#x9678;&#x8ECD;&#x533B;&#x7642;&#x30B3;&#x30DE;&#x30F3;&#x30C9;&#x306B;&#x30D0;&#x30E9;&#x30F3;&#x30B9; &#x30B9;&#x30B3;&#x30A2;&#x30AB;&#x30FC;&#x30C9;&#x3068;&#x770B;&#x8B77;&#x30B1;&#x30A2;&#x5916;&#x6765;&#x3084;&#x5165;&#x9662;&#x306E;&#x305F;&#x3081;&#x306E;&#x8907;&#x6570;&#x306E;&#x4EBA;&#x4E8B;&#x3068;&#x4EBA;&#x6750;&#x30D3;&#x30B8;&#x30CD;&#x30B9; &#x30D7;&#x30ED;&#x30BB;&#x30B9;&#x306E;&#x7D99;&#x7D9A;&#x7684;&#x306A;&#x7DAD;&#x6301;&#x3092;&#x5FC5;&#x8981;&#x3068;&#x3059;&#x308B;&#x8981;&#x4EF6;&#x3092;&#x627F;&#x8A8D;&#x3057;&#x307E;&#x3059;&#x3002;&#x3053;&#x306E;&#x30B7;&#x30B9;&#x30C6;&#x30E0;&#x306F;&#x73FE;&#x5728; TRICARE &#x7BA1;&#x7406;&#x6D3B;&#x52D5;&#x3068;&#x3057;&#x3066;&#x3001;&#x4E2D;&#x9593;&#x306E;&#x30DE;&#x30EB;&#x30C1;&#x30B5;&#x30FC;&#x30D3;&#x30B9; &#x30FB; &#x30BD;&#x30EA;&#x30E5;&#x30FC;&#x30B7;&#x30E7;&#x30F3;&#x306B;&#x3088;&#x3063;&#x3066;&#x6D3B;&#x7528;&#x3055;&#x308C;&#x3066;&#x3001;24 &#x306E;&#x9678;&#x8ECD;&#x533B;&#x7642;&#x65BD;&#x8A2D;&#x3067;&#x4F7F;&#x7528;&#x3055;&#x308C;&#x3066;&#x3044;&#x307E;&#x3059;&#x3002;&#x8ECD;&#x306E;&#x610F;&#x601D;&#x6C7A;&#x5B9A;&#x8005;&#x306B; WMSNi &#x3067;&#x4F7F;&#x7528;&#x3055;&#x308C;&#x308B;&#x30E1;&#x30BD;&#x30C3;&#x30C9;&#x3092;&#x4ECB;&#x3057;&#x3066;&#x63D0;&#x4F9B;&#x3055;&#x308C;&#x308B;&#x8A3C;&#x62E0;&#x306B;&#x57FA;&#x3065;&#x304F;&#x60C5;&#x5831;&#x30B7;&#x30B9;&#x30C6;&#x30E0;&#x306E;&#x30E9;&#x30A4;&#x30D5; &#x30B5;&#x30A4;&#x30AF;&#x30EB;&#x5168;&#x4F53;&#x306B;&#x9678;&#x8ECD;&#x533B;&#x7642;&#x30B3;&#x30DE;&#x30F3;&#x30C9;&#x5168;&#x4F53;&#x306B;&#x4E0D;&#x53EF;&#x6B20;&#x306B;&#x306A;&#x308A;&#x307E;&#x3059;&#x3002;

&#x54C1;&#x8CEA;&#x306E;&#x60A3;&#x8005;&#x30B1;&#x30A2;&#x306E;&#x30A2;&#x30A6;&#x30C8;&#x30AB;&#x30E0;&#x3078;&#x306E;&#x672C;&#x8CEA;&#x7684;&#x306A;&#x8CA2;&#x732E;&#x3092;&#x770B;&#x8B77;&#x3092;&#x6E2C;&#x5B9A;&#x3057;&#x307E;&#x3059;&#x3002;

&#xC791;&#xC5C5; &#xBD80;&#xD558; &#xAD00;&#xB9AC; &#xC2DC;&#xC2A4;&#xD15C; &#xAC04;&#xD638; (WMSN)&#xC5D0; &#xB300; &#xD55C; &#xCF54;&#xC5B4; &#xADFC;&#xCC98;&#xC5D0; &#xC2E4;&#xC2DC;&#xAC04;&#xC73C;&#xB85C;, &#xD3EC;&#xAD04;&#xC801;&#xC778; &#xAC04;&#xD638; &#xC758;&#xC0AC; &#xACB0;&#xC815;&#xC5D0; &#xB300; &#xD55C; &#xC791;&#xC5C5; &#xBC0F; &#xC778;&#xB825; &#xB370;&#xC774;&#xD130; &#xBAA8;&#xB4E0; &#xC218;&#xC900;&#xC5D0;&#xC11C; 25 &#xB144; &#xC774;&#xC0C1;&#xC5D0; &#xB300; &#xD55C; &#xC81C;&#xACF5;&#xAC00; &#xC721;&#xAD70; &#xC758;&#xB8CC; &#xBD80;&#xC11C; &#xBE44;&#xC988;&#xB2C8;&#xC2A4; &#xC2DC;&#xC2A4;&#xD15C;&#xC785;&#xB2C8;&#xB2E4;. &#xC721;&#xAD70; &#xC778;&#xB825; &#xC694;&#xAD6C; &#xC0AC;&#xD56D; &#xBC0F; &#xBB38;&#xC11C; &#xAE30;&#xAD00; &#xC778;&#xB825; &#xC778;&#xB825; &#xD45C;&#xC900; &#xC2DC;&#xC2A4;&#xD15C; (&#xC790;&#xB3D9;&#xC73C;&#xB85C; &#xC778;&#xB825; &#xD3C9;&#xAC00; &#xBAA8;&#xB378; &#xB0B4;&#xC5D0;&#xC11C; &#xC785;&#xC6D0; &#xD658;&#xC790; &#xBAA8;&#xB4C8;)&#xC5D0; WMSN &#xAE30;&#xC900;&#xC5D0;&#xC11C; &#xB370;&#xC774;&#xD130;&#xB97C; &#xCC44;&#xC6C1;&#xB2C8;&#xB2E4;. &#xD604;&#xC7AC; &#xC2DC;&#xC2A4;&#xD15C; &#xC791;&#xC5C5; &#xBD80;&#xD558; &#xAD00;&#xB9AC; &#xC2DC;&#xC2A4;&#xD15C; &#xAC04;&#xD638; &#xC778;&#xD130;&#xB137; (WMSNi)&#xC740; &#xD604;&#xB300;&#xD654; &#xBC0F; &#xC5D4;&#xD130;&#xD504;&#xB77C;&#xC774;&#xC988; &#xC218;&#xC900;&#xC5D0;&#xC11C; &#xD1B5;&#xD569;&#xC744; &#xC704;&#xD55C; &#xCD94;&#xAC00; &#xAE30;&#xB2A5;&#xC744; &#xD544;&#xC694;&#xB85C; &#xD558;&#xB294; &#xC784;&#xC2DC; &#xC194;&#xB8E8;&#xC158;. &#xD655;&#xC7A5; &#xC784;&#xBB34; &#xC721;&#xAD70; &#xC0AC;&#xB839;&#xBD80; &#xC758;&#xB8CC;&#xC5D0; WMSNi &#xC9C0;&#xC6D0; &#xC804;&#xB7B5;&#xC801; &#xC774;&#xB2C8;&#xC154;&#xD2F0;&#xBE0C; &#xADE0;&#xD615; &#xC131;&#xACFC; &#xAE30;&#xB85D;&#xD45C; &#xBC0F; &#xC785;&#xC6D0; &#xBC0F; &#xC678;&#xB798; &#xD658;&#xC790; &#xAC04; &#xBCD1;&#xC5D0; &#xB300; &#xD55C; &#xC5EC;&#xB7EC; &#xC778;&#xC0AC;&#xC640; &#xC778;&#xB825; &#xBE44;&#xC988;&#xB2C8;&#xC2A4; &#xD504;&#xB85C;&#xC138;&#xC2A4;&#xC5D0; &#xB300; &#xD55C; &#xC9C0;&#xC18D;&#xC801;&#xC778;&#xB41C; &#xBCF4;&#xAE09;&#xD488;&#xC744; &#xD544;&#xC694;&#xB85C; &#xB300; &#xD55C; &#xC694;&#xAD6C; &#xC0AC;&#xD56D;&#xC744; &#xC2B9;&#xC778; &#xD569;&#xB2C8;&#xB2E4;. &#xC774; &#xC2DC;&#xC2A4;&#xD15C;&#xACFC; TRICARE &#xAD00;&#xB9AC; &#xD65C;&#xB3D9; &#xC911;&#xAC04; &#xBA40;&#xD2F0; &#xC11C;&#xBE44;&#xC2A4; &#xC194;&#xB8E8;&#xC158;&#xC73C;&#xB85C; &#xD65C;&#xC6A9; &#xB418; &#xACE0; &#xD604;&#xC7AC; 24 &#xC721;&#xAD70; &#xC758;&#xB8CC; &#xC2DC;&#xC124;&#xC5D0;&#xC11C; &#xC0AC;&#xC6A9; &#xB418; &#xACE0; &#xC788;&#xB2E4;. &#xC721;&#xAD70; &#xC758;&#xC0AC; &#xACB0;&#xC815;&#xC790;&#xC5D0; &#xAC8C; &#xC81C;&#xACF5; &#xD558;&#xB294; Wmsni&#xC5D0;&#xC11C; &#xC0AC;&#xC6A9; &#xD558;&#xB294; &#xBC29;&#xBC95;&#xC744; &#xD1B5;&#xD574; &#xC785;&#xC99D; &#xAE30;&#xBC18; &#xC815;&#xBCF4; &#xC721;&#xAD70; &#xC0AC;&#xB839;&#xBD80; &#xC758;&#xB8CC; &#xC2DC;&#xC2A4;&#xD15C;&#xC758; &#xC218;&#xBA85; &#xC8FC;&#xAE30; &#xC804;&#xCCB4;&#xC5D0; &#xAC78;&#xCCD0; &#xD544;&#xC218;&#xC801;&#xC778; &#xAC83;&#xC785;&#xB2C8;&#xB2E4;.

&#xAC04;&#xD638; &#xC9C8; &#xD658;&#xC790; &#xCE58;&#xB8CC; &#xACB0;&#xACFC;&#xC5D0; &#xD544;&#xC218;&#xC801;&#xC778; &#xAE30;&#xC5EC;&#xB97C; &#xCE21;&#xC815;.

Sistema de gesti&oacute;n de la carga de trabajo de enfermer&iacute;a (WMSN) es un sistema de negocio del departamento m&eacute;dico del ej&eacute;rcito que ha proporcionado cerca de en tiempo real, datos de la carga de trabajo y mano de obra para la toma de decisiones de enfermer&iacute;a integral en todos los niveles por m&aacute;s de 25 a&ntilde;os. La Agencia de la documentaci&oacute;n y requisitos de mano de obra del ej&eacute;rcito rellena los datos de WMSN en el sistema de normas de personal de mano de obra (m&oacute;dulo de hospitalizaci&oacute;n en el modelo de evaluaci&oacute;n de personal automatizado). El actual sistema, sistema de gesti&oacute;n de la carga de trabajo de enfermer&iacute;a Internet (WMSNi), es una soluci&oacute;n provisional que requiere funcionalidades adicionales para la modernizaci&oacute;n y la integraci&oacute;n a nivel de empresa. Las expansi&oacute;n de las misiones y aprobado los requisitos para WMSNi iniciativas de apoyo estrat&eacute;gicas en el comando m&eacute;dico del ej&eacute;rcito balanced scorecard y requieren mantenimiento continuo de m&uacute;ltiples procesos de negocio de personal y recursos humanos para pacientes internos y ambulatorios de atenci&oacute;n de enfermer&iacute;a. Este sistema es actualmente ser apalancado por la actividad de gesti&oacute;n de TRICARE como soluci&oacute;n provisional multiservicio y est&aacute; siendo usado en 24 instalaciones de tratamiento m&eacute;dico de ej&eacute;rcito. La informaci&oacute;n basada en evidencia proporcionada a los tomadores de decisi&oacute;n del ej&eacute;rcito a trav&eacute;s de los m&eacute;todos utilizados en la WMSNi ser&aacute; esencial a trav&eacute;s del comando m&eacute;dico del ej&eacute;rcito a lo largo del ciclo de vida del sistema.

Medici&oacute;n de enfermer&iacute;a contribuciones esenciales a resultados de calidad asistencial.

Astrocyte elevated gene-1 (AEG-1) and c-Myc are overexpressed in human hepatocellular carcinoma (HCC) functioning as oncogenes. AEG-1 is transcriptionally regulated by c-Myc, and AEG-1 itself induces c-Myc by activating the Wnt/β-catenin-signaling pathway. We now document the cooperation of AEG-1 and c-Myc in promoting hepatocarcinogenesis by analyzing hepatocyte-specific transgenic mice expressing either AEG-1 (albumin [Alb]/AEG-1), c-Myc (Alb/c-Myc), or both (Alb/AEG-1/c-Myc). Wild-type and Alb/AEG-1 mice did not develop spontaneous HCC. Alb/c-Myc mice developed spontaneous HCC without distant metastasis, whereas Alb/AEG-1/c-Myc mice developed highly aggressive HCC with frank metastasis to the lungs. Induction of carcinogenesis by N-nitrosodiethylamine significantly accelerated the kinetics of tumor formation in all groups. However, in Alb/AEG-1/c-Myc, the effect was markedly pronounced with lung metastasis. In vitro analysis showed that Alb/AEG-1/c-Myc hepatocytes acquired increased proliferation and transformative potential with sustained activation of prosurvival and epithelial-mesenchymal transition-signaling pathways. RNA-sequencing analysis identified a unique gene signature in livers of Alb/AEG-1/c-Myc mice that was not observed when either AEG-1 or c-Myc was overexpressed. Specifically, Alb/AEG-1/c-Myc mice overexpressed maternally imprinted noncoding RNAs (ncRNAs), such as Rian, Meg-3, and Mirg, which are implicated in hepatocarcinogenesis. Knocking down these ncRNAs significantly inhibited proliferation and invasion by Alb/AEG-1/c-Myc hepatocytes.

Astrocyte elevated gene-1 and c-Myc cooperate to promote hepatocarcinogenesis in mice.

Activation of IGF signaling is a major oncogenic event in diverse cancers, including hepatocellular carcinoma (HCC). In this setting, the insulin-like growth factor binding protein IGFBP7 inhibits IGF signaling by binding the IGF1 receptor (IGF1R), functioning as a candidate tumor suppressor. IGFBP7 abrogates tumors by inhibiting angiogenesis and inducing cancer-specific senescence and apoptosis. Here, we report that Igfbp7-deficient mice exhibit constitutively active IGF signaling, presenting with proinflammatory and immunosuppressive microenvironments and spontaneous liver and lung tumors occurring with increased incidence in carcinogen-treated subjects. Igfbp7 deletion increased proliferation and decreased senescence of hepatocytes and mouse embryonic fibroblasts, effects that were blocked by treatment with IGF1 receptor inhibitor. Significant inhibition of genes regulating immune surveillance was observed in Igfbp7(-/-) murine livers, which was associated with a marked inhibition in antigen cross-presentation by Igfbp7(-/-) dendritic cells. Conversely, IGFBP7 overexpression inhibited growth of HCC cells in syngeneic immunocompetent mice. Depletion of CD4(+) or CD8(+) T lymphocytes abolished this growth inhibition, identifying it as an immune-mediated response. Our findings define an immune component of the pleiotropic mechanisms through which IGFBP7 suppresses HCC. Furthermore, they offer a genetically based preclinical proof of concept for IGFBP7 as a therapeutic target for immune management of HCC. Cancer Res; 77(15); 4014-25. ©2017 AACR.

Dawn Garcia

Greehey Children's Cancer Research Institute

University of Texas Health Science Center at San Antonio

Methyl-binding DNA capture Sequencing for Patient Tissues

Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation

Dawn Garcia

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Greehey Children's Cancer Research Institute

University of Texas Health Science Center at San Antonio

Methyl-binding DNA capture Sequencing for Patient Tissues

Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation

Greehey Children's Cancer Research Institute