Pierre-Olivier Couraud

Characterization of genetically modified human retinal pigment epithelial cells developed for in vitro and transplantation studies.

Microvilli-like structures are associated with the internalization of virulent capsulated Neisseria meningitidis into vascular endothelial cells.

How do extracellular pathogens cross the blood-brain barrier?

Lymphocyte trafficking through the blood-brain barrier is dependent on endothelial cell heterotrimeric G-protein signaling.

Human adipose cells express CD4, CXCR4, and CCR5 [corrected] receptors: a new target cell type for the immunodeficiency virus-1?

Lymphocyte migration into the central nervous system: implication of ICAM-1 signalling at the blood-brain barrier.

Changes in cytoskeletal and tight junctional proteins correlate with decreased permeability induced by dexamethasone in cultured rat brain endothelial cells.

JAML, a novel protein with characteristics of a junctional adhesion molecule, is induced during differentiation of myeloid leukemia cells.

Intracellular domain of brain endothelial intercellular adhesion molecule-1 is essential for T lymphocyte-mediated signaling and migration.

Development and characterization of immortalized cerebral endothelial cell lines.

Trans-inactivation of receptor tyrosine kinases by novel angiotensin II AT2 receptor-interacting protein, ATIP.

Rat brain endothelial cell lines for the study of blood-brain barrier permeability and transport functions.

Invasion of endothelial cells by Neisseria meningitidis requires cortactin recruitment by a phosphoinositide-3-kinase/Rac1 signalling pathway triggered by the lipo-oligosaccharide.

[Endothelin-1, angiotensin II and cancer].

Neisseria meningitidis infection of human endothelial cells interferes with leukocyte transmigration by preventing the formation of endothelial docking structures.

Junctional expression of the prion protein PrPC by brain endothelial cells: a role in trans-endothelial migration of human monocytes.

TAL-1/SCL and its partners E47 and LMO2 up-regulate VE-cadherin expression in endothelial cells.

A functional in vitro model of rat blood-brain barrier for molecular analysis of efflux transporters.

Reactive oxygen species alter brain endothelial tight junction dynamics via RhoA, PI3 kinase, and PKB signaling.

Immortalized human brain endothelial cells and flow-based vascular modeling: a marriage of convenience for rational neurovascular studies.

PECAM-1 engagement counteracts ICAM-1-induced signaling in brain vascular endothelial cells.

Human blood-brain barrier disruption by retroviral-infected lymphocytes: role of myosin light chain kinase in endothelial tight-junction disorganization.

[Functions of prion protein PrPc].

Regulation of cerebral endothelial cell morphology by extracellular calcium.

Differential effects of hydrocortisone and TNFalpha on tight junction proteins in an in vitro model of the human blood-brain barrier.

Simvastatin protects against amyloid beta and HIV-1 Tat-induced promoter activities of inflammatory genes in brain endothelial cells.

Pcbs and tight junction expression.

Molecular mechanism of systemic delivery of neural precursor cells to the brain: assembly of brain endothelial apical cups and control of transmigration by CD44.

Caveolin-1 regulates human immunodeficiency virus-1 Tat-induced alterations of tight junction protein expression via modulation of the Ras signaling.

Hyperosmotic stress induces Axl activation and cleavage in cerebral endothelial cells.

Tissue-type plasminogen activator is a regulator of monocyte diapedesis through the brain endothelial barrier.

Alteration of blood-brain barrier integrity by retroviral infection.

The human brain endothelial cell line hCMEC/D3 as a human blood-brain barrier model for drug transport studies.

Expression and transcriptional regulation of ABC transporters and cytochromes P450 in hCMEC/D3 human cerebral microvascular endothelial cells.

The blood-brain barrier in brain homeostasis and neurological diseases.

JAM-L-mediated leukocyte adhesion to endothelial cells is regulated in cis by alpha4beta1 integrin activation.

ABC transporters, cytochromes P450 and their main transcription factors: expression at the human blood-brain barrier.

Expression of ADAM-17, TIMP-3 and fractalkine in the human adult brain endothelial cell line, hCMEC/D3, following pro-inflammatory cytokine treatment.

Meningococcal type IV pili recruit the polarity complex to cross the brain endothelium.

Role of KCNMA1 gene in breast cancer invasion and metastasis to brain.

Peripheral blood CD4+ T lymphocytes from multiple sclerosis patients are characterized by higher PSGL-1 expression and transmigration capacity across a human blood-brain barrier-derived endothelial cell line.

Modulation of blood-brain barrier permeability by neutrophils: in vitro and in vivo studies.

Immortalized human brain endothelial cell line HCMEC/D3 as a model of the blood-brain barrier facilitates in vitro studies of central nervous system infection by Cryptococcus neoformans.

Induced secretion of beta-hexosaminidase by human brain endothelial cells: a novel approach in Sandhoff disease?

Identification of peptide ligands for targeting to the blood-brain barrier.

IL8 and CXCL13 are potent chemokines for the recruitment of human neural precursor cells across brain endothelial cells.

Design of fluorescently tagged poly(alkyl cyanoacrylate) nanoparticles for human brain endothelial cell imaging.

A novel vascular targeting strategy for brain-derived endothelial cells using a TCR mimic antibody.

Matrix metalloproteinase 2 (MMP-2) degrades soluble vasculotropic amyloid-beta E22Q and L34V mutants, delaying their toxicity for human brain microvascular endothelial cells.

Metabolic acidosis induced by Plasmodium falciparum intraerythrocytic stages alters blood-brain barrier integrity.

Plasmodium falciparum adhesion on human brain microvascular endothelial cells involves transmigration-like cup formation and induces opening of intercellular junctions.

Role of astrocytic leptin receptor subtypes on leptin permeation across hCMEC/D3 human brain endothelial cells.

Surface characteristics of nanoparticles determine their intracellular fate in and processing by human blood-brain barrier endothelial cells in vitro.

Evaluation of soluble junctional adhesion molecule-A as a biomarker of human brain endothelial barrier breakdown.

Uptake and permeability studies of BBB-targeting immunoliposomes using the hCMEC/D3 cell line.

Tyrosine phosphorylation of VE-cadherin and claudin-5 is associated with TGF-β1-induced permeability of centrally derived vascular endothelium.

Differential expression of the bone and the liver tissue non-specific alkaline phosphatase isoforms in brain tissues.

Secreted factors from brain endothelial cells maintain glioblastoma stem-like cell expansion through the mTOR pathway.

Human brain endothelial cells are responsive to adenosine receptor activation.

Development of a three-dimensional, all-human in vitro model of the blood-brain barrier using mono-, co-, and tri-cultivation Transwell models.

Transcriptomic and quantitative proteomic analysis of transporters and drug metabolizing enzymes in freshly isolated human brain microvessels.

Functionalization with ApoE-derived peptides enhances the interaction with brain capillary endothelial cells of nanoliposomes binding amyloid-beta peptide.

Insights into the pathogenesis of systemic sclerosis based on the gene expression profile of progenitor-derived endothelial cells.

Insights into the putative catechin and epicatechin transport across blood-brain barrier.

Invading basement membrane matrix is sufficient for MDA-MB-231 breast cancer cells to develop a stable in vivo metastatic phenotype.

Purine receptors and Ca(2+) signalling in the human blood-brain barrier endothelial cell line hCMEC/D3.

A differential proteomic approach identifies structural and functional components that contribute to the differentiation of brain capillary endothelial cells.

The metastatic microenvironment: brain-residing melanoma metastasis and dormant micrometastasis.

Angiogenic biomarkers predict the occurrence of digital ulcers in systemic sclerosis.

Hepatitis C virus infects the endothelial cells of the blood-brain barrier.

Lamotrigine is a substrate for OCT1 in brain endothelial cells.

Guanine nucleotide-binding protein Gαi2: a new partner of claudin-5 that regulates tight junction integrity in human brain endothelial cells.

The involvement of a Na⁺- and Cl⁻-dependent transporter in the brain uptake of amantadine and rimantadine.

Expression and localization of claudins-3 and -12 in transformed human brain endothelium.

[Transendothelial migration through the neurovascular unit: "a cup to cross the barrier"].

The metastatic microenvironment: Brain-derived soluble factors alter the malignant phenotype of cutaneous and brain-metastasizing melanoma cells.

Angiotensin II facilitates breast cancer cell migration and metastasis.

CCL2 disrupts the adherens junction: implications for neuroinflammation.

Cell-penetrating anti-GFAP VHH and corresponding fluorescent fusion protein VHH-GFP spontaneously cross the blood-brain barrier and specifically recognize astrocytes: application to brain imaging.

Uptake and cytotoxicity of citrate-coated gold nanospheres: Comparative studies on human endothelial and epithelial cells.

Localization microscopy (SPDM) reveals clustered formations of P-glycoprotein in a human blood-brain barrier model.

Transcellular targeting of fiber- and hexon-modified adenovirus vectors across the brain microvascular endothelial cells in vitro.

Immortalized human cerebral microvascular endothelial cells maintain the properties of primary cells in an in vitro model of immune migration across the blood brain barrier.

Quantitative targeted absolute proteomic analysis of transporters, receptors and junction proteins for validation of human cerebral microvascular endothelial cell line hCMEC/D3 as a human blood-brain barrier model.

Tight junctions at the blood brain barrier: physiological architecture and disease-associated dysregulation.

Cooperation between β- and γ-cytoplasmic actins in the mechanical regulation of endothelial microparticle formation.

The brain microvascular endothelium supports T cell proliferation and has potential for alloantigen presentation.

TWEAK/Fn14 pathway modulates properties of a human microvascular endothelial cell model of blood brain barrier.

Functional expression of a proton-coupled organic cation (H+/OC) antiporter in human brain capillary endothelial cell line hCMEC/D3, a human blood-brain barrier model.

The angiogenic gene profile of circulating endothelial progenitor cells from ischemic stroke patients.

High-density lipoprotein-based therapy reduces the hemorrhagic complications associated with tissue plasminogen activator treatment in experimental stroke.

Glial-cell-mediated re-induction of the blood-brain barrier phenotype in brain capillary endothelial cells: a differential gel electrophoresis study.

Overexpression of CD44 in neural precursor cells improves trans-endothelial migration and facilitates their invasion of perivascular tissues in vivo.

The hCMEC/D3 cell line as a model of the human blood brain barrier.

Targeting endothelial CD146 attenuates neuroinflammation by limiting lymphocyte extravasation to the CNS.

Effects of Aggregatibacter actinomycetemcomitans leukotoxin on endothelial cells.

A multi-system approach assessing the interaction of anticonvulsants with P-gp.

Somatostatin preserved blood brain barrier against cytokine induced alterations: possible role in multiple sclerosis.

Temozolomide down-regulates P-glycoprotein in human blood-brain barrier cells by disrupting Wnt3 signaling.

Temporal profile of matrix metalloproteinases and their inhibitors in a human endothelial cell culture model of cerebral ischemia.

Wnt activation of immortalized brain endothelial cells as a tool for generating a standardized model of the blood brain barrier in vitro.

Immature mesenchymal stem cell-like pericytes as mediators of immunosuppression in human malignant glioma.

Paracrine signalling of inflammatory cytokines from an in vitro blood brain barrier model upon exposure to polymeric nanoparticles.

The Wnt/planar cell polarity signaling pathway contributes to the integrity of tight junctions in brain endothelial cells.

Instruction of circulating endothelial progenitors in vitro towards specialized blood-brain barrier and arterial phenotypes.

Drug-induced trafficking of p-glycoprotein in human brain capillary endothelial cells as demonstrated by exposure to mitomycin C.

CXCL1/CXCL8 (GROα/IL-8) in human diabetic ketoacidosis plasma facilitates leukocyte recruitment to cerebrovascular endothelium in vitro.

Endotoxin-induced monocytic microparticles have contrasting effects on endothelial inflammatory responses.

Oxidative and pro-inflammatory impact of regular and denicotinized cigarettes on blood brain barrier endothelial cells: is smoking reduced or nicotine-free products really safe?

Primary angiitis of the central nervous system: description of the first fifty-two adults enrolled in the French cohort of patients with primary vasculitis of the central nervous system.

Pathogenic Neisseria meningitidis utilizes CD147 for vascular colonization.

The cross-talk between canonical and non-canonical Wnt-dependent pathways regulates P-glycoprotein expression in human blood-brain barrier cells.

Nanoparticle formulation improves the anticonvulsant effect of clonazepam on the pentylenetetrazole-induced seizures: behavior and electroencephalogram.

Synthesis and in vitro evaluation of BBB permeability, tumor cell uptake, and cytotoxicity of a series of carboranylporphyrin conjugates.

Edaravone protects against methylglyoxal-induced barrier damage in human brain endothelial cells.

The metastatic microenvironment: Claudin-1 suppresses the malignant phenotype of melanoma brain metastasis.

The poorly membrane permeable antipsychotic drugs amisulpride and sulpiride are substrates of the organic cation transporters from the SLC22 family.

In vitro modeling of the neurovascular environment by coculturing adult human brain endothelial cells with human neural stem cells.

Permeability of PEGylated immunoarsonoliposomes through in vitro blood brain barrier-medulloblastoma co-culture models for brain tumor therapy.

Carrier-mediated cocaine transport at the blood-brain barrier as a putative mechanism in addiction liability.

Astrocytes facilitate melanoma brain metastasis via secretion of IL-23.

Contribution of pannexin 1 and connexin 43 hemichannels to extracellular calcium-dependent transport dynamics in human blood-brain barrier endothelial cells.

Differential permissivity of human cerebrovascular endothelial cells to enterovirus infection and specificities of serotype EV-A71 in crossing an in vitro model of the human blood-brain barrier.

Vemurafenib resistance selects for highly malignant brain and lung-metastasizing melanoma cells.

Development of a solvent-free analytical method for paracetamol quantitative determination in Blood Brain Barrier in vitro model.

Phenotypic variation in Aicardi-Goutières syndrome explained by cell-specific IFN-stimulated gene response and cytokine release.

Aryl hydrocarbon receptor regulates CYP1B1 but not ABCB1 and ABCG2 in hCMEC/D3 human cerebral microvascular endothelial cells after TCDD exposure.

Inflammatory response of endothelial cells to a human endogenous retrovirus associated with multiple sclerosis is mediated by TLR4.

Serotype O18 avian pathogenic and neonatal meningitis Escherichia coli strains employ similar pathogenic strategies for the onset of meningitis.

The hunt for brain Aβ oligomers by peripherally circulating multi-functional nanoparticles: Potential therapeutic approach for Alzheimer disease.

Immuno-analysis of microparticles: probing at the limits of detection.

Solid lipid nanoparticles as a vehicle for brain-targeted drug delivery: two new strategies of functionalization with apolipoprotein E.

Assessment of electrophile damage in a human brain endothelial cell line utilizing a clickable alkyne analog of 2-chlorohexadecanal.

Early alterations of Hedgehog signaling pathway in vascular endothelial cells after peripheral nerve injury elicit blood-nerve barrier disruption, nerve inflammation, and neuropathic pain development.

Transthyretin participates in beta-amyloid transport from the brain to the liver--involvement of the low-density lipoprotein receptor-related protein 1?

In vitro models of the blood-brain barrier: An overview of commonly used brain endothelial cell culture models and guidelines for their use.

Human T-Lymphotropic Virus Type 1-Induced Overexpression of Activated Leukocyte Cell Adhesion Molecule (ALCAM) Facilitates Trafficking of Infected Lymphocytes through the Blood-Brain Barrier.

Prolonged Morphine Exposure Induces Increased Firm Adhesion in an in Vitro Model of the Blood-Brain Barrier.

Methylphenidate-triggered ROS generation promotes caveolae-mediated transcytosis via Rac1 signaling and c-Src-dependent caveolin-1 phosphorylation in human brain endothelial cells.

[Research funding: a bottleneck for economic growth].