effects-blast-induced-neurotrauma-on-pressurized-rodent-middle

Effects of Blast-induced Neurotrauma on Pressurized Rodent Middle Cerebral Arteries

Though there have been studies on the histopathological and behavioral effects of blast exposure, fewer have been dedicated to blast&#39;s cerebral ...

University of Texas Medical Branch

university of texas medical branch

To determine the effects of mild blast-induced traumatic brain injury (bTBI), several groups of rats were subjected to blast injury or sham injury in a compressed air-driven shock tube. The effects of bTBI on relative cerebral perfusion (laser Doppler flowmetry [LDF]), and mean arterial blood pressure (MAP) cerebral vascular resistance were measured for 2 h post-bTBI. Dilator responses to reduced intravascular pressure were measured in isolated middle cerebral arterial (MCA) segments, ex vivo, 30 and 60 min post-bTBI. Neuronal injury was assessed (Fluoro-Jade C [FJC]) 24 and 48 h post-bTBI. Neurological outcomes (beam balance and walking tests) and working memory (Morris water maze [MWM]) were assessed 2 weeks post-bTBI. Because impact TBI (i.e., non-blast TBI) is often associated with reduced cerebral perfusion and impaired cerebrovascular function in part because of the generation of reactive oxygen and nitrogen species such as peroxynitrite (ONOO-), the effects of the administration of the ONOO- scavenger, penicillamine methyl ester (PenME), on cerebral perfusion and cerebral vascular resistance were measured for 2 h post-bTBI. Mild bTBI resulted in reduced relative cerebral perfusion and MCA dilator responses to reduced intravascular pressure, increases in cerebral vascular resistance and in the numbers of FJC-positive cells in the brain, and significantly impaired working memory. PenME administration resulted in significant reductions in cerebral vascular resistance and a trend toward increased cerebral perfusion, suggesting that ONOO- may contribute to blast-induced cerebral vascular dysfunction.

Uylissa A. Rodriguez

Charles R. Allen Research Laboratories,
Department of Anesthesiology,
The Moody Project for Translational Traumatic Brain Injury Research,
Charles R. Allen Research Laboratories, Department of Anesthesiology

University of Texas Medical Branch

Effects of Blast-induced Neurotrauma on Pressurized Rodent Middle Cerebral Arteries

Uylissa A. Rodriguez

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Charles R. Allen Research Laboratories,Department of Anesthesiology,The Moody Project for Translational Traumatic Brain Injury Research,Charles R. Allen Research Laboratories, Department of Anesthesiology

University of Texas Medical Branch

Effects of Blast-induced Neurotrauma on Pressurized Rodent Middle Cerebral Arteries

Charles R. Allen Research Laboratories,
Department of Anesthesiology,
The Moody Project for Translational Traumatic Brain Injury Research,
Charles R. Allen Research Laboratories, Department of Anesthesiology