experimental-viral-infection-adult-mosquitoes-oral-feeding

Experimental Viral Infection in Adult Mosquitoes by Oral Feeding and Microinjection

Mosquito-borne viruses (MBVs), which are infectious pathogens to vertebrates, are spread by many mosquito species, posing a severe threat to public ...

Chinese Academy of Sciences

University of Chinese Academy of Sciences

Mosquito-specific flaviviruses comprise a group of insect-specific viruses with a single positive RNA, which can affect the duplication of mosquito-borne viruses and the life growth of mosquitoes, and which have the potential to be developed as a vaccine platform for mosquito-borne viruses. In this study, a strain of mosquito flavivirus (MFV) YN15-283-02 was detected in  collected from Yunnan, China. The isolation of the purified MFV YN15-283-02 from cell culture failed, and the virus was then rescued by an infectious clone. To study the biological features of MFV YN15-283-02 in vitro and in vivo, electron microscopy, phylogenetic tree, and viral growth kinetic analyses were performed in both cell lines and mosquitoes. The rescued MFV (rMFV) YN15-283-02 duplicated and reached a peak in C6/36 cells at 6 d.p.i. with approximately 2 × 10 RNA copies/μL (RNA to cell ratio of 0.1), but without displaying a cytopathic effect. In addition, the infection rate for the rMFV in  show a low level in both larvae (≤15%) and adult mosquitoes (≤12%).

Shunlong Wang

Key Laboratory of Special Pathogens and Biosafety,
Wuhan Institute of Virology,
Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology

Chinese Academy of Sciences

Experimental Viral Infection in Adult Mosquitoes by Oral Feeding and Microinjection

Shunlong Wang

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Key Laboratory of Special Pathogens and Biosafety,Wuhan Institute of Virology,Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology

Chinese Academy of Sciences

Experimental Viral Infection in Adult Mosquitoes by Oral Feeding and Microinjection

Key Laboratory of Special Pathogens and Biosafety,
Wuhan Institute of Virology,
Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology