Robert O.J. Weinzierl

A recombinant RNA polymerase II-like enzyme capable of promoter-specific transcription.

A fully recombinant system for activator-dependent archaeal transcription.

Direct modulation of RNA polymerase core functions by basal transcription factors.

Modulation of RNA polymerase core functions by basal transcription factor TFB/TFIIB.

The RNA polymerase factory: a robotic in vitro assembly platform for high-throughput production of recombinant protein complexes.

Bridge helix and trigger loop perturbations generate superactive RNA polymerases.

Nanomechanical constraints acting on the catalytic site of cellular RNA polymerases.

The linker domain of basal transcription factor TFIIB controls distinct recruitment and transcription stimulation functions.

The nucleotide addition cycle of RNA polymerase is controlled by two molecular hinges in the Bridge Helix domain.

Cation-Ï€ interactions induce kinking of a molecular hinge in the RNA polymerase bridge-helix domain.

Activity map of the Escherichia coli RNA polymerase bridge helix.

Revealing the functions of TFIIB.

The Bridge Helix of RNA polymerase acts as a central nanomechanical switchboard for coordinating catalysis and substrate movement.