S'identifier

Chang Gung Memorial Hospital

7 ARTICLES PUBLISHED IN JoVE

Medicine

Assessment of Right Ventricular Structure and Function in Mouse Model of Pulmonary Artery Constriction by Transthoracic Echocardiography

Hui-Wen Cheng^*1,2, Sudeshna Fisch^*1, Susan Cheng¹, Michael Bauer¹, Soeun Ngoy¹, Yiling Qiu¹, Jian Guan¹, Shikha Mishra¹, Christopher Mbah¹, Ronglih Liao¹

¹Cardiac Muscle Research Labratory, Cardiovascular Division, Brigham and Women’s Hospital, Harvard Medical School, ²Cardiovascular Department, Chang Gung Memorial Hospital

Right ventricle (RV) dysfunction is critical to the pathogenesis of cardiovascular disease, yet limited methodologies are available for its evaluation. Recent advances in ultrasound imaging provide a noninvasive and accurate option for longitudinal RV study. Herein, we detail a step-by-step echocardiographic method using a murine model of RV pressure overload.

Bioengineering

Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells

Yen-Li Wang^1,2, Adrienne Hong³, Tzung-Hai Yen^1,4,5,6, Hsiang-Hsi Hong^1,7,8

¹Chang Gung University, ²Department of Periodontics, Chang Gung Memorial Hospital, ³California Northstate University College of Medicine, ⁴Department of Nephrology, Clinical Poison Center, Chang Gung Memorial Hospital, ⁵Kidney Research Center, Chang Gung Memorial Hospital, ⁶Center for Tissue Engineering, Chang Gung Memorial Hospital, ⁷Department of Periodontics, Chang Gung Memorial Hospital, ⁸College of Oral Medicine, Taipei Medical University

We present a protocol to investigate the mRNA expression biomarkers of periosteum-derived cells (PDCs) induced by vitamin C (vitamin C) and 1,25-dihydroxy vitamin D [1,25-(OH)₂D₃]. In addition, we evaluate the ability of PDCs to differentiate into osteocytes, chondrocytes, and adipocytes.

Medicine

Phase Contrast Magnetic Resonance Imaging in the Rat Common Carotid Artery

Shao-Chieh Chiu¹, Shih-Ting Hsu¹, Chiun-Wei Huang¹, Wu-Chung Shen², Shin-Lei Peng²

¹Center for Advanced Molecular Imaging and Translation, Chang Gung Memorial Hospital, ²Department of Biomedical Imaging and Radiological Science, China Medical University

The overall goal of this procedure is to measure the blood flow in the rat common carotid artery by using noninvasive phase contrast magnetic resonance imaging.

Neuroscience

Dorsal Root Ganglia Isolation and Primary Culture to Study Neurotransmitter Release

Ya-Tin Lin¹, Jin-Chung Chen^1,2,3

¹Graduate Institute of Biomedical Sciences, Department of Physiology and Pharmacology, Chang Gung University, ²Healthy Aging Research Center, Chang Gung University, ³Neuroscience Research Center, Chang Gung Memorial Hospital

Dorsal root ganglia (DRG) primary cultures are frequently used to study physiological functions or pathology-related events in sensory neurons. Here, we demonstrate the use of lumbar DRG cultures to detect the release of neurotransmitters after neuropeptide FF receptor type 2 stimulation with a selective agonist.

Bioengineering

A Custom Multiphoton Microscopy Platform for Live Imaging of Mouse Cornea and Conjunctiva

Yueh-Feng Wu¹, Rai-Teng Ye¹, Ming-Kai Pan^2,3, Sung-Jan Lin^*1,3,4,5, Hsin-Yuan Tan^*6,7

¹Department of Biomedical Engineering, National Taiwan University, ²Institute of Pharmacology, College of Medicine, National Taiwan University, ³Molecular Imaging Center, National Taiwan University, ⁴Department of Dermatology, National Taiwan University Hospital, and College of Medicine, ⁵Research Center for Developmental Biology and Regenerative Medicine, National Taiwan University, ⁶Department of Ophthalmology, Chang Gung Memorial Hospital, ⁷College of Medicine, Chang Gung University

Presented here is a multiphoton microscopic platform for live mouse ocular surface imaging. Fluorescent transgenic mouse enables the visualization of cell nuclei, cell membranes, nerve fibers and capillaries within the ocular surface. Non-linear second harmonic generation signals derived from collagenous structures provide label-free imaging for stromal architectures.

Medicine

Development of a Novel Task-oriented Rehabilitation Program using a Bimanual Exoskeleton Robotic Hand

Yi-Mei Chen¹, Szu-Shen Lai¹, Yu-Cheng Pei^2,3,4,5, Chia-Ju Hsieh¹, Wei-Han Chang¹

¹Department of Physical Medicine and Rehabilitation, Chang Gung Memorial Hospital at Taoyuan, ²Department of Physical Medicine and Rehabilitation, Chang Gung Memorial Hospital at Linkou, ³School of Medicine, Chang Gung University, ⁴Center for Vascularized Composite Allotransplantation, Chang Gung Memorial Hospital, ⁵Healthy Aging Research Center, Chang Gung University

This study reports the development of a novel robot-assisted task-oriented program for hand rehabilitation. The developmental process consists of experiments using both healthy subjects and subjects who have had a stroke and suffered from subsequent motor control dysfunction.

Cancer Research

Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres

Chun-Chia Cheng¹, Po-Jui Hsu⁴, Zong-Lin Sie¹, Fang-Hsin Chen^1,2,3

¹Radiation Biology Research Center, Institute for Radiological Research, Chang Gung University, Chang Gung Memorial Hospital at Linkou, ²Department of Medical Imaging and Radiological Sciences, Chang Gung University, ³Department of Radiation Oncology, Chang Gung Memorial Hospital, ⁴Department of Laboratory Medicine, Mackay Memorial Hospital

Presented here is a protocol to discover the overexpressed driver genes maintaining the established cancer stem-like cells derived from colorectal HT29 cells. RNAseq with available bioinformatics was performed to investigate and screen gene expression networks for elucidating a potential mechanism involved in the survival of targeted tumor cells.