Results: Representative Tumorsphere Protein and Gene Expression Analyses
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Conclusion
Transcription
Our protocol utilized RNA-Seq and bioinformatical tools to quickly uncover the overexpressed driver genes that contribute to the formation of stem-like colorectal tumorspheres. This protocol is used to screen a differentially expressed gene and si
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Presented here is a protocol to discover the overexpressed driver genes maintaining the established cancer stem-like cells derived from colorectal HT29 cells. RNAseq with available bioinformatics was performed to investigate and screen gene expression networks for elucidating a potential mechanism involved in the survival of targeted tumor cells.