S'identifier

Cambridge Institute of Science

1 ARTICLES PUBLISHED IN JoVE

Neuroscience

The "Brain Milking" Method for the Isolation of Neural Stem Cells and Oligodendrocyte Progenitor Cells from Live Rats

Dimitrios Dimitrakopoulos¹, Chrisitna Dimitriou¹, Freyja McClenahan², Robin J. M. Franklin^2,3, Ilias Kazanis¹

¹Laboratory of Developmental Biology, Department of Biology, University of Patras, ²Wellcome Trust-MRC Cambridge Stem Cell Institute, University of Cambridge, ³Altos Labs, Cambridge Institute of Science

A method for the isolation of neural stem cells and oligodendrocyte progenitor cells from the brains of live rats is presented here in experimental detail. It allows multiple collections of these cells from the same animals without compromising their well-being.