Meizhou People's Hospital
2 ARTICLES PUBLISHED IN JoVE
Biology
Generation and Characterization of Rat Uterus Organoids from Rat Endometrial Epithelial Stem Cells
Man Yang 1, Qinghua Liu 2, Yong Chen 2, Jun Li 2, Wen He 1,3
1Meizhou Clinical Institute of Shantou University Medical College, 2Department of Pathology, Meizhou People's Hospital, 3Guangdong Engineering Technological Research Center of Clinical Molecular Diagnosis and Antibody Drugs, Meizhou Academy of Medical Sciences, Meizhou People's Hospital
Here, we present a protocol to isolate and culture rat endometrial epithelial stem cells (reESCs), generating rat endometrial organoids. This method facilitates in vitro studies of endometrial diseases, enabling gene editing and other cellular manipulations.
Medicine
The Immediate Partial Removal of Cumulus-Oocyte Complexes: A Refined Approach for Rapid Observation of In Vitro Fertilization
Yantian Zheng *1, Wen He *1,2, Siping Chen 1, Honggan Yi 1, Man Yang 1
1Department of Reproductive Medicine, Meizhou People's Hospital, 2Guangdong Engineering Technological Research Center of Clinical Molecular Diagnosis and Antibody Drugs, Meizhou Academy of Medical Sciences, Meizhou People's Hospital
Here, we present an optimized method for promptly removing a portion of cumulus-oocyte complexes following egg retrieval to expedite the IVF observation process, reducing the time required for granulosa cell removal, minimizing oocyte exposure to external elements, and does not hinder embryo development, ultimately improving the efficiency of IVF laboratory procedures.
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