Accedi

Meizhou People's Hospital

2 ARTICLES PUBLISHED IN JoVE

Biology

Generation and Characterization of Rat Uterus Organoids from Rat Endometrial Epithelial Stem Cells

Man Yang¹, Qinghua Liu², Yong Chen², Jun Li², Wen He^1,3

¹Meizhou Clinical Institute of Shantou University Medical College, ²Department of Pathology, Meizhou People's Hospital, ³Guangdong Engineering Technological Research Center of Clinical Molecular Diagnosis and Antibody Drugs, Meizhou Academy of Medical Sciences, Meizhou People's Hospital

Here, we present a protocol to isolate and culture rat endometrial epithelial stem cells (reESCs), generating rat endometrial organoids. This method facilitates in vitro studies of endometrial diseases, enabling gene editing and other cellular manipulations.

Medicine

The Immediate Partial Removal of Cumulus-Oocyte Complexes: A Refined Approach for Rapid Observation of In Vitro Fertilization

Yantian Zheng^*1, Wen He^*1,2, Siping Chen¹, Honggan Yi¹, Man Yang¹

¹Department of Reproductive Medicine, Meizhou People's Hospital, ²Guangdong Engineering Technological Research Center of Clinical Molecular Diagnosis and Antibody Drugs, Meizhou Academy of Medical Sciences, Meizhou People's Hospital

Here, we present an optimized method for promptly removing a portion of cumulus-oocyte complexes following egg retrieval to expedite the IVF observation process, reducing the time required for granulosa cell removal, minimizing oocyte exposure to external elements, and does not hinder embryo development, ultimately improving the efficiency of IVF laboratory procedures.