S'identifier

Kutztown University of Pennsylvania

2 ARTICLES PUBLISHED IN JoVE

JoVE Journal

Identification of Intracellular Signaling Events Induced in Viable Cells by Interaction with Neighboring Cells Undergoing Apoptotic Cell Death

Snezana Vujicic^*1,2, Lanfei Feng^*1,2, Angelika Antoni³, Joyce Rauch⁴, Jerrold S. Levine^1,2,5

¹Section of Nephrology, Department of Medicine, University of Illinois at Chicago, ²Section of Nephrology, Department of Medicine, Jesse Brown Veterans Affairs Medical Center, ³Department of Biology, Kutztown University of Pennsylvania, ⁴Division of Rheumatology, Department of Medicine, Research Institute of the McGill University Health Centre, ⁵Department of Microbiology & Immunology, University of Illinois at Chicago

Here, we present a protocol for the determination of intracellular signaling events induced in viable cells by physical interaction with adjacent dead or dying cells. The protocol focuses on signaling events induced by receptor-mediated recognition of the dead cells, as opposed to their phagocytic uptake or release of soluble mediators.

Biology

Live-cell Imaging of Fungal Cells to Investigate Modes of Entry and Subcellular Localization of Antifungal Plant Defensins

Kazi T. Islam¹, Dilip M. Shah¹, Kaoutar El-Mounadi²

¹Donald Danforth Plant Science Center, ²Department of Biology, Kutztown University of Pennsylvania

Plant defensins play an important role in plant defense against pathogens. For effective use of these antifungal peptides as antifungal agents, understanding their modes of action (MOA) is critical. Here, a live-cell imaging method is described to study critical aspects of the MOA of these peptides.