S'identifier

university of kaiserslautern

2 ARTICLES PUBLISHED IN JoVE

Biology

A Protocol for the Identification of Protein-protein Interactions Based on ¹⁵N Metabolic Labeling, Immunoprecipitation, Quantitative Mass Spectrometry and Affinity Modulation

Stefan Schmollinger^1,2, Daniela Strenkert^1,2, Vittoria Offeddu^1,2, André Nordhues^1,2, Frederik Sommer^1,2, Michael Schroda^1,2

¹Max Planck Institute of Molecular Plant Physiology, ²University of Kaiserslautern

We present a variation of the QUICK (QUantitative Immunoprecipitation Combined with Knockdown) approach that was introduced previously to distinguish between true and false protein-protein interactions. Our approach is based on ^15Nmetabolic labeling, the modulation of affinities of protein-protein interactions by the presence/absence of ATP, immunoprecipitation, and quantitative mass spectrometry.

Bioengineering

Aqueous Droplets Used as Enzymatic Microreactors and Their Electromagnetic Actuation

Huschyar Al-Kaidy¹, Kai Kuthan¹, Thomas Hering¹, Nils Tippkötter^1,2

¹Institute of Bioprocess Engineering, University of Kaiserslautern, ²Bioprocess Engineering, University of Applied Sciences, FH Aachen

Lab-in-a-drop reaction systems allow the versatile implementation of complex reactions in a microfluidic scale. An automated actuation platform consisting of a 3 x 3 matrix of electromagnetic coils was developed and successfully used to merge two 10 µL microreactors and thereby initiate an enzymatic reaction in the resulting liquid marbles.