University of Wuerzburg
2 ARTICLES PUBLISHED IN JoVE
Neuroscience
Lectin-based Isolation and Culture of Mouse Embryonic Motoneurons
Rebecca Conrad 1, Sibylle Jablonka 2, Teresa Sczepan 1, Michael Sendtner 2, Stefan Wiese 1, Alice Klausmeyer 1
1Institute for Cellmorphology and molecular Neurobiology, Group for Cellbiology, Ruhr-University Bochum, 2Institute for Clinical Neurobiology, University of Wuerzburg
An alternative way of isolating mouse embryonic motoneurons from the spinal cord is described. The method takes into account the fact that lectin can bind to the low affinity nerve growth factor receptor p75NTR. This lectin-based preplating allows a purification similar to that with a specific antibody against the p75NTR.
Biology
Direct Imaging of ER Calcium with Targeted-Esterase Induced Dye Loading (TED)
Samira Samtleben 1, Juliane Jaepel 1,2, Caroline Fecher 1, Thomas Andreska 1, Markus Rehberg 3, Robert Blum 1
1Institute for Clinical Neurobiology, University of Wuerzburg, 2Department of Synapses - Circuits - Plasticity, Max Planck Institute of Neurobiology, Martinsried, 3Walter Brendel Centre of Experimental Medicine, Ludwig-Maximilians University of Munich
Targeted-esterase induced dye loading (TED) supports the analysis of intracellular calcium store dynamics by fluorescence imaging. The method bases on targeting of a recombinant Carboxylesterase to the endoplasmic reticulum (ER), where it improves the local unmasking of synthetic low-affinity Ca2+ indicator dyes in the ER lumen.
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