University of Brescia
2 ARTICLES PUBLISHED IN JoVE
Developmental Biology
Stable and Efficient Genetic Modification of Cells in the Adult Mouse V-SVZ for the Analysis of Neural Stem Cell Autonomous and Non-autonomous Effects
Eva Porlan 1, Beatriz Martí-Prado 2,3, Antonella Consiglio 4,5, Isabel Fariñas 2,3
1Cell Division and Cancer Group, Spanish National Cancer Research Centre (CNIO), 2Centro de Investigaciones Biomédicas en Red sobre Enfermedades Neurodegenerativas (CIBERNED), 3Departmento de Biologìa Celular, Universidad de Valencia, 4Institut de Biomedicina de la Universitat de Barcelona (IBUB), 5Department of Molecular and Translational Medicine, Fibroblast Reprogramming Unit, University of Brescia
Here we describe a procedure based on the use of lentiviral particles for the long-term genetic modification of neural stem cells and/or their adjacent ependymal cells in the adult ventricular-subventricular neurogenic niche which allows the separate analysis of cell autonomous and non-autonomous, niche-dependent effects on neural stem cells.
Medicine
Learning Modern Laryngeal Surgery in a Dissection Laboratory
Erika Crosetti 1, Marco Fantini 1, Davide Lancini 2, Andrea Manca 1, Giovanni Succo 1,3
1Head and Neck Oncology Unit, Candiolo Cancer Institute, FPO-IRCCS, 2Department of Otorhinolaryngology - Head and Neck Surgery, University of Brescia, 3Department of Oncology, University of Turin
The purpose of this paper is to illustrate how to organize a reproducible laboratory for laryngeal surgery on affordable and closely similar animal laryngeal models in order to improve anatomical and surgical knowledge and skills.
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