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University of Brescia

2 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Stable and Efficient Genetic Modification of Cells in the Adult Mouse V-SVZ for the Analysis of Neural Stem Cell Autonomous and Non-autonomous Effects

Eva Porlan¹, Beatriz Martí-Prado^2,3, Antonella Consiglio^4,5, Isabel Fariñas^2,3

¹Cell Division and Cancer Group, Spanish National Cancer Research Centre (CNIO), ²Centro de Investigaciones Biomédicas en Red sobre Enfermedades Neurodegenerativas (CIBERNED), ³Departmento de Biologìa Celular, Universidad de Valencia, ⁴Institut de Biomedicina de la Universitat de Barcelona (IBUB), ⁵Department of Molecular and Translational Medicine, Fibroblast Reprogramming Unit, University of Brescia

Here we describe a procedure based on the use of lentiviral particles for the long-term genetic modification of neural stem cells and/or their adjacent ependymal cells in the adult ventricular-subventricular neurogenic niche which allows the separate analysis of cell autonomous and non-autonomous, niche-dependent effects on neural stem cells.

Medicine

Learning Modern Laryngeal Surgery in a Dissection Laboratory

Erika Crosetti¹, Marco Fantini¹, Davide Lancini², Andrea Manca¹, Giovanni Succo^1,3

¹Head and Neck Oncology Unit, Candiolo Cancer Institute, FPO-IRCCS, ²Department of Otorhinolaryngology - Head and Neck Surgery, University of Brescia, ³Department of Oncology, University of Turin

The purpose of this paper is to illustrate how to organize a reproducible laboratory for laryngeal surgery on affordable and closely similar animal laryngeal models in order to improve anatomical and surgical knowledge and skills.