Cell Cycle-specific Measurement of γH2AX and Apoptosis After Genotoxic Stress by Flow CytometryRamon Lopez Perez 1,2, Franziska Münz 1,2, Jonas Kroschke 1,2, Jannek Brauer 1,2, Nils H. Nicolay 1,2,3, Peter E. Huber 1,2
1CCU Molecular and Radiation Oncology, German Cancer Research Center, 2Department of Radiation Oncology, Heidelberg University Hospital, 3Department of Radiation Oncology, Freiburg University Medical Center
The presented method combines the quantitative analysis of DNA double-strand breaks (DSBs), cell cycle distribution and apoptosis to enable cell cycle-specific evaluation of DSB induction and repair as well as the consequences of repair failure.
