Methanol Independent Expression by Pichia Pastoris Employing De-repression TechnologiesJasmin Elgin Fischer 1, Anna-Maria Hatzl 2, Astrid Weninger 2, Christian Schmid 2, Anton Glieder 1,2
1bisy e.U., 2Institute of Molecular Biotechnology, Technical University of Graz
This method for the first time describes reliable experimental procedures for efficient inducible methanol-free recombinant protein production in Pichia pastoris (Komagataella phaffii), employing the carbon source regulated promoters PDCand PDF in small scale experiments while cultivation parameters can be monitored online, and a constant glycerol feed is applied.
