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1 ARTICLES PUBLISHED IN JoVE

Bioengineering

Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies

Jasmin Elgin Fischer¹, Anna-Maria Hatzl², Astrid Weninger², Christian Schmid², Anton Glieder^1,2

¹bisy e.U., ²Institute of Molecular Biotechnology, Technical University of Graz

This method for the first time describes reliable experimental procedures for efficient inducible methanol-free recombinant protein production in Pichia pastoris (Komagataella phaffii), employing the carbon source regulated promoters P_DCand P_DF in small scale experiments while cultivation parameters can be monitored online, and a constant glycerol feed is applied.