Mount Allison University
2 ARTICLES PUBLISHED IN JoVE
Biology
Adenovirus-mediated Genetic Removal of Signaling Molecules in Cultured Primary Mouse Embryonic Fibroblasts
Steve P. Hawley *1, Melanie K. B. Wills *1, Nina Jones 1
1Department of Molecular and Cellular Biology, University of Guelph
In this video we use an adenovirus carrying the Cre recombinase gene to infect primary mouse embryonic fibroblasts carrying a floxed Rac1 allele.
Biology
Detecting the Lyme Disease Spirochete, Borrelia Burgdorferi, in Ticks Using Nested PCR
Melanie K. B. Wills 1, Andrea M. Kirby 1, Vett K. Lloyd 1
1Department of Biology, Mount Allison University
Nested PCR is a sensitive, specific, and straightforward technique that can be applied to tick DNA extracts to probe for Borrelia burgdorferi, the causative agent of Lyme disease. The initial PCR experiment uses gene-specific primers to generate long amplicons, which then become templates for a subsequent reaction using internal primers.
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