S'identifier

Tohoku University Graduate School of Medicine

3 ARTICLES PUBLISHED IN JoVE

Biology

The Method of Rodent Whole Embryo Culture using the Rotator-type Bottle Culture System

¹Division of Developmental Neuroscience, United Centers for Advanced Research and Translational Medicine (ART), Tohoku University Graduate School of Medicine, ²The Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Corporation (JST)

Whole embryo culture technique allows us to culture mouse and rat embryos ex vivo condition during limited periods corresponding to midgestation stages. In this video protocol, we demonstrate our standard procedures of rat whole embryo culture after E12.5 using the rotator-type bottle culture system.

Immunology and Infection

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing

Lin Sun¹, Naoko Kono², Hiroyuki Toh³, Hanbing Xue¹, Kaori Sano^4,5, Tadaki Suzuki⁴, Akira Ainai⁴, Yasuko Orba⁶, Junya Yamagishi^7,8, Hideki Hasegawa^4,5, Yoshimasa Takahashi⁹, Shigeyuki Itamura², Kazuo Ohnishi^9,10

¹Graduate School of Life and Environmental Sciences, University of Tsukuba, ²Center for Influenza Virus Research, National Institute of Infectious Diseases, ³School of Science and Technology, Kwansei Gakuin University, ⁴Department of Pathology, National Institute of Infectious Diseases, ⁵Division of Infectious Diseases Pathology, Department of Global Infectious Diseases, Tohoku University Graduate School of Medicine, ⁶Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, ⁷Division of Collaboration and Education, Research Center for Zoonosis Control, Hokkaido University, ⁸Global Station for Zoonosis Control, GI-CoRE, Hokkaido University, ⁹Department of Immunology, National Institute of Infectious Diseases, ¹⁰Faculty of Life and Environmental Sciences, University of Tsukuba

Here, we describe protocols for the analysis and visualization of the structure and constitution of whole antibody repertoires. This involves the acquisition of vast sequences of antibody RNA using next-generation sequencing.

Medicine

Measurement of Myocardial Lactate Production for Diagnosis of Coronary Microvascular Spasm

Jun Takahashi¹, Akira Suda¹, Satoshi Yasuda¹, Hiroaki Shimokawa¹

¹Department of Cardiovascular Medicine, Tohoku University Graduate School of Medicine

Myocardial lactate production (coronary arterial-venous difference in serum lactate level) during coronary spasm provocation testing is considered as a highly sensitive marker that reflects acetylcholine-induced myocardial ischemia due to microvascular spasm. This article presents the procedures to assess myocardial lactate production for the diagnosis of coronary microvascular spasm.