Max Planck Institute of Biophysics
2 ARTICLES PUBLISHED IN JoVE
Biology
Introduction to Solid Supported Membrane Based Electrophysiology
Andre Bazzone 1, Wagner Steuer Costa 2, Markus Braner 1, Octavian Călinescu 1, Lina Hatahet 1, Klaus Fendler 1
1Department of Biophysical Chemistry, Max Planck Institute of Biophysics, 2Buchmann Institute for Molecular Life Sciences, Goethe University Frankfurt
Here we present an electrophysiological method based on solid supported membranes with focus on its applications for the characterization of electrogenic membrane transporters.
Biology
Visualization of ATP Synthase Dimers in Mitochondria by Electron Cryo-tomography
Karen M. Davies 1, Bertram Daum 1, Vicki A. M. Gold 1, Alexander W. Mühleip 1, Tobias Brandt 1, Thorsten B. Blum 1, Deryck J. Mills 1, Werner Kühlbrandt 1
1Department of Structural Biology, Max Planck Institute of Biophysics
We present a protocol of how to collect and process electron cryo-tomograms of whole mitochondria. The technique provides detailed insights into the structure, function, and organization of large membrane protein complexes in native biological membranes.
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