Accedi

Max Planck Institute of Biophysics

2 ARTICLES PUBLISHED IN JoVE

Biology

Introduction to Solid Supported Membrane Based Electrophysiology

Andre Bazzone¹, Wagner Steuer Costa², Markus Braner¹, Octavian Călinescu¹, Lina Hatahet¹, Klaus Fendler¹

¹Department of Biophysical Chemistry, Max Planck Institute of Biophysics, ²Buchmann Institute for Molecular Life Sciences, Goethe University Frankfurt

Here we present an electrophysiological method based on solid supported membranes with focus on its applications for the characterization of electrogenic membrane transporters.

Biology

Visualization of ATP Synthase Dimers in Mitochondria by Electron Cryo-tomography

Karen M. Davies¹, Bertram Daum¹, Vicki A. M. Gold¹, Alexander W. Mühleip¹, Tobias Brandt¹, Thorsten B. Blum¹, Deryck J. Mills¹, Werner Kühlbrandt¹

¹Department of Structural Biology, Max Planck Institute of Biophysics

We present a protocol of how to collect and process electron cryo-tomograms of whole mitochondria. The technique provides detailed insights into the structure, function, and organization of large membrane protein complexes in native biological membranes.