Simultaneous pH Measurement in Endocytic and Cytosolic Compartments in Living Cells using Confocal MicroscopyFabrice Lucien 1, Kelly Harper 1, Pierre-Paul Pelletier 1, Leonid Volkov 2, Claire M. Dubois 1
1Department of Pediatrics, Faculty of Medicine and Health Sciences, Université de Sherbrooke, 2Biophotonics Core Facility, Centre de Recherche Clinique Etienne-Le Bel
Several methods are available for measuring intracellular pH but very few of these allow simultaneous measurement of cytoplasmic and organellar pH. Here, we describe in detail a rapid and accurate methodology to simultaneously measure cytoplasmic and vesicular pH by ratiometric imaging of living cells.
