S'identifier

CRCHUM and University of Montreal

2 ARTICLES PUBLISHED IN JoVE

Medicine

A Magnetic Microbead Occlusion Model to Induce Ocular Hypertension-Dependent Glaucoma in Mice

Yoko A. Ito^*1, Nicolas Belforte^*1, Jorge L. Cueva Vargas¹, Adriana Di Polo¹

¹Department of Neuroscience, CRCHUM and University of Montreal

Here, we present a protocol to induce ocular hypertension in the murine eye that results in the loss of retinal ganglion cells as observed in glaucoma. Magnetic microbeads are injected into the anterior chamber and attracted to the iridocorneal angle using a magnet to block the outflow of aqueous humour.

Immunology and Infection

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Manuel Flores Molina^1,2, Thomas Fabre^1,2, Aurélie Cleret-Buhot¹, Geneviève Soucy^1,3, Liliane Meunier^1,4, Mohamed N. Abdelnabi^1,2, Nicolas Belforte^1,5, Simon Turcotte^1,4,6, Naglaa H. Shoukry^1,4,7

¹Centre de Recherche du Centre hospitalier de l'Université de Montréal (CRCHUM), ²Département de Microbiologie, Infectiologie et Immunologie, Faculté de Médecine, Université de Montréal, ³Département de Pathologie et Biologie Cellulaire, Faculté de Médecine, Université de Montréal, ⁴Institut du Cancer de Montréal, ⁵Département de Neurosciences, Faculté de Médecine, Université de Montréal, ⁶Département de Chirurgie, Faculté de Médecine, Université de Montréal, ⁷Département de Médecine, Faculté de médecine, Université de Montréal

Here, we describe a simple and accessible strategy for visualizing, quantifying, and mapping immune cells in formalin-fixed paraffin-embedded tumor tissue sections. This methodology combines existing imaging and digital analysis techniques with the purpose of expanding the multiplexing capability and multiparameter analysis of imaging assays.