Accedi

CRCHUM and University of Montreal

2 ARTICLES PUBLISHED IN JoVE

image

Medicine

A Magnetic Microbead Occlusion Model to Induce Ocular Hypertension-Dependent Glaucoma in Mice
Yoko A. Ito *1, Nicolas Belforte *1, Jorge L. Cueva Vargas 1, Adriana Di Polo 1
1Department of Neuroscience, CRCHUM and University of Montreal

Here, we present a protocol to induce ocular hypertension in the murine eye that results in the loss of retinal ganglion cells as observed in glaucoma. Magnetic microbeads are injected into the anterior chamber and attracted to the iridocorneal angle using a magnet to block the outflow of aqueous humour.

image

Immunology and Infection

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment
Manuel Flores Molina 1,2, Thomas Fabre 1,2, Aurélie Cleret-Buhot 1, Geneviève Soucy 1,3, Liliane Meunier 1,4, Mohamed N. Abdelnabi 1,2, Nicolas Belforte 1,5, Simon Turcotte 1,4,6, Naglaa H. Shoukry 1,4,7
1Centre de Recherche du Centre hospitalier de l'Université de Montréal (CRCHUM), 2Département de Microbiologie, Infectiologie et Immunologie, Faculté de Médecine, Université de Montréal, 3Département de Pathologie et Biologie Cellulaire, Faculté de Médecine, Université de Montréal, 4Institut du Cancer de Montréal, 5Département de Neurosciences, Faculté de Médecine, Université de Montréal, 6Département de Chirurgie, Faculté de Médecine, Université de Montréal, 7Département de Médecine, Faculté de médecine, Université de Montréal

Here, we describe a simple and accessible strategy for visualizing, quantifying, and mapping immune cells in formalin-fixed paraffin-embedded tumor tissue sections. This methodology combines existing imaging and digital analysis techniques with the purpose of expanding the multiplexing capability and multiparameter analysis of imaging assays.

JoVE Logo

Riservatezza

Condizioni di utilizzo

Politiche

Ricerca

Didattica

CHI SIAMO

Copyright © 2024 MyJoVE Corporation. Tutti i diritti riservati