Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291Jenessa A. Winston 1, Rajani Thanissery 1, Stephanie A. Montgomery 2, Casey M. Theriot 1
1Department of Population Health and Pathobiology, North Carolina State University College of Veterinary Medicine, 2Department of Pathology and Laboratory Medicine, Lineberger Comprehensive Cancer Center, University of North Carolina School of Medicine
This protocol outlines the cefoperazone mouse model of Clostridium difficile infection (CDI) using a clinically-relevant and genetically-tractable strain, R20291. Emphasis on clinical disease monitoring, C. difficile bacterial enumeration, toxin cytotoxicity, and histopathological changes throughout CDI in a mouse model are detailed in the protocol.
