Hillman Cancer Center
2 ARTICLES PUBLISHED IN JoVE
Biology
Isolation and Culture of Adult Mouse Cardiomyocytes for Cell Signaling and in vitro Cardiac Hypertrophy
Daxiang Li 1, Jian Wu 1, Yan Bai 1, Xiaochen Zhao 2, Lijun Liu 1
1Department of Biochemistry and Cancer Biology, University of Toledo College of Medicine and Life Sciences, 2Department of Physiology and Pharmacology, University of Toledo College of Medicine and Life Sciences
We describe a reliable method for isolation of adult mouse cardiomyocytes. This protocol yields a consistent result for the culture of functional adult cardiomyocytes from a variety of genetically modified mice.
Cancer Research
A Model for Perineural Invasion in Head and Neck Squamous Cell Carcinoma
Phillip Huyett 1, Mark Gilbert 1, Lijun Liu 2, Robert L. Ferris 2, Seungwon Kim 1
1Department of Otolaryngology, University of Pittsburgh Medical Center, 2University of Pittsburgh Cancer Institute, Hillman Cancer Center
Perineural invasion (PNI) is a common feature of head and neck squamous cell carcinoma (HNSCC), conferring lower survival rates. Its mechanisms are poorly understood. Utilizing neurites generated from murine dorsal root ganglia confined to a semisolid matrix, the pathways involved in the PNI of HNSCC cell lines can be investigated.
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