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Hillman Cancer Center

2 ARTICLES PUBLISHED IN JoVE

Biology

Isolation and Culture of Adult Mouse Cardiomyocytes for Cell Signaling and in vitro Cardiac Hypertrophy

Daxiang Li¹, Jian Wu¹, Yan Bai¹, Xiaochen Zhao², Lijun Liu¹

¹Department of Biochemistry and Cancer Biology, University of Toledo College of Medicine and Life Sciences, ²Department of Physiology and Pharmacology, University of Toledo College of Medicine and Life Sciences

We describe a reliable method for isolation of adult mouse cardiomyocytes. This protocol yields a consistent result for the culture of functional adult cardiomyocytes from a variety of genetically modified mice.

Cancer Research

A Model for Perineural Invasion in Head and Neck Squamous Cell Carcinoma

Phillip Huyett¹, Mark Gilbert¹, Lijun Liu², Robert L. Ferris², Seungwon Kim¹

¹Department of Otolaryngology, University of Pittsburgh Medical Center, ²University of Pittsburgh Cancer Institute, Hillman Cancer Center

Perineural invasion (PNI) is a common feature of head and neck squamous cell carcinoma (HNSCC), conferring lower survival rates. Its mechanisms are poorly understood. Utilizing neurites generated from murine dorsal root ganglia confined to a semisolid matrix, the pathways involved in the PNI of HNSCC cell lines can be investigated.