S'identifier

University of Oklahoma Health Science Center

2 ARTICLES PUBLISHED IN JoVE

JoVE Journal

An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants

Yuhui Chen¹, Xianfu Wang², Shunfei Lu³, Hongcheng Wang², Shibo Li², Rujin Chen¹

¹Laboratory of Plant Genetics and Development, Noble Research Institute, ²Genetics Laboratory, University of Oklahoma Health Science Center, ³Medicine and Health School, Li Shui University

This protocol provides experimental steps and information about reagents, equipment, and analysis tools for researchers who are interested in carrying out whole genome array-based comparative genomic hybridization (CGH) analysis of copy number variations in plants.

Immunology and Infection

Real-Time Measurement of the Mitochondrial Bioenergetic Profile of Neutrophils

Sunitha Pulikkot¹, Meng Zhao^2,3, Zhichao Fan¹

¹Department of Immunology, School of Medicine, UConn Health, ²Arthritis and Clinical Immunology Program, Oklahoma Medical Research Foundation, ³Department of Microbiology and Immunology, University of Oklahoma Health Science Center

We describe stepwise protocols measuring the mitochondrial respiration of mouse and human neutrophils and HL60 cells using the metabolic extracellular flux analyzer.