Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after VaccinationKatherine Schultheis 1, Hubert Schaefer 2, Holly M. Pugh 1, Bryan S. Yung 1, Janet Oh 1, Jacklyn Nguyen 1, Laurent Humeau 1, Kate E. Broderick 1, Trevor R.F. Smith 1
1Inovio Pharmaceuticals, Inc, 2Mycotic and Parasitic Agents and Mycobacteria, Robert Koch Institute
The development of the interferon-gamma ELISpot assay for guinea pig PBMCs allows the characterization of T-cell responses in this highly relevant model for studying infectious diseases. We have applied the assay to measure T cell responses associated with intradermal delivery of DNA vaccines.
