A Layered Mounting Method for Extended Time-Lapse Confocal Microscopy of Whole Zebrafish EmbryosSanat Upadhyay 1, Leoncio Vergara 2, Pranjali Shah 2, Jan-Åke Gustafsson 3,4, Ioannis Kakadiaris 1,4, Maria Bondesson 5
1Computational Biomedicine Lab, Texas Institute of Measurement Evaluation and Statistics, University of Houston, 2Institute of Biosciences and Technology, Texas A&M Health Science Center, 3Department of Biology and Biochemistry, Center for Nuclear Receptors and Cell Signaling, University of Houston, 4Department of Biosciences and Nutrition, Novum, Karolinska Institutet, 5Department of Intelligent Systems Engineering, Indiana University
This article describes a method to mount fragile zebrafish embryos for extended time-lapse confocal microscopy. This low-cost method is easy to perform using regular glass-bottom microscopy dishes for imaging on any inverted microscope. The mounting is performed in layers of agarose at different concentrations.
