Fish Husbandry and Preparation of Solutions and Embryos
1:44
Mounting in Agarose and Optimization of Agarose Solution
3:38
Time-lapse Imaging
6:07
Results: Extended Time-lapse Imaging of Zebrafish Embryos
7:54
Conclusion
Transcription
We have developed a mounting method for time-lapse imaging that allows for unrestricted growth of fragile zebrafish embryos and at the same time keeps them in a completely fixed position. This mounting method is fast, easy, and cheap and it works
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This article describes a method to mount fragile zebrafish embryos for extended time-lapse confocal microscopy. This low-cost method is easy to perform using regular glass-bottom microscopy dishes for imaging on any inverted microscope. The mounting is performed in layers of agarose at different concentrations.