S'identifier

American Red Cross, National Headquarters

2 ARTICLES PUBLISHED IN JoVE

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Medicine

The Polyvinyl Alcohol Sponge Model Implantation
Desirae L. Deskins 1,2, Shidrokh Ardestani 1,2, Pampee P. Young 1,2,3
1Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, 2The Department of Veterans Affairs Medical Center, 3Internal Medicine, Vanderbilt University School of Medicine

A useful tool to analyze the effects of drugs, growth factors, and/or manipulated cells in an animal model of wound repair is described. This technique utilizes the properties of a polyvinyl alcohol (PVA) sponge to deliver and contain the desired treatment and also provide a platform to be excised and analyzed.

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Biology

Isolation and Characterization of Adult Cardiac Fibroblasts and Myofibroblasts
Meiling Melzer 1, David Beier 1, Pampee P. Young 1,2, Sarika Saraswati 1
1Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, 2American Red Cross, National Headquarters

Obtaining a pure population of fibroblasts is crucial to studying their role in wound repair and fibrosis. Described here is a detailed method to isolate fibroblasts and myofibroblasts from uninjured and injured mouse hearts followed by characterization of their purity and functionality by immunofluorescence, RTPCR, fluorescence-assisted cell sorting, and collagen gel contraction.

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