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National Institute for Quantum and Radiological Science and Technology

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Behavior

Calcium Imaging in Freely Behaving Caenorhabditis elegans with Well-Controlled, Nonlocalized Vibration
Kazuki Shigyou *1, Haruka Maeoka *1, Ryuji Igarashi 2,3,4, Takuma Sugi 1
1Program of Biomedical Science, Graduate School of Integrated Sciences for Life, Hiroshima University, 2Institute for Quantum Life Science, National Institute for Quantum and Radiological Science and Technology, 3National Institute for Radiological Sciences, National Institute for Quantum and Radiological Science and Technology, 4JST, PRESTO

Reported here is a system for calcium imaging in freely behaving Caenorhabditis elegans with well-controlled, nonlocalized vibration. This system allows researchers to evoke nonlocalized vibrations with well-controlled properties at nano-scale displacement and to quantify calcium currents during responses of C. elegans to the vibrations.

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