Co-culture of Glioblastoma Stem-like Cells on Patterned Neurons to Study Migration and Cellular InteractionsJoris Guyon 1, Pierre-Olivier Strale 2,3, Irati Romero-Garmendia 4, Andreas Bikfalvi 1, Vincent Studer *2, Thomas Daubon *4
1University Bordeaux, INSERM, LAMC, U1029, 33600, Pessac, France, 2Univ. Bordeaux, CNRS, Interdisciplinary Institute for Neuroscience, IINS, UMR 5297, Bordeaux, France, 3Alvéole, Bordeaux, France, 4University Bordeaux, CNRS, IBGC, UMR5095, 33000, Bordeaux, France
Here, we present an easy-to-use co-culture assay to analyze glioblastoma (GBM) migration on patterned neurons. We developed a macro in FiJi software for easy quantification of GBM cell migration on neurons, and observed that neurons modify GBM cell invasive capacity.
