Preparation of Embryonic Rat Hippocampal Neurons and GBM Cells for Co-Culture
3:22
Live Cell Imaging
3:58
Neuronal Network Analysis
5:48
Single-Cell Motility Analysis
7:08
Multiple-Cell Motility Analysis
7:57
Results: Representative Analysis of GBM Migration on Patterned Neurons
9:15
Conclusion
Transcription
Glioblastomas are devastating brain tumors with a high invasive profile. This co-culture system mimics glioblastoma cells migration on neurons to recapitulate one of the invasive routes observed in patients. The geometry and composition of our co-
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Here, we present an easy-to-use co-culture assay to analyze glioblastoma (GBM) migration on patterned neurons. We developed a macro in FiJi software for easy quantification of GBM cell migration on neurons, and observed that neurons modify GBM cell invasive capacity.