2D and 3D Human Induced Pluripotent Stem Cell-Based Models to Dissect Primary Cilium Involvement during Neocortical DevelopmentLucile Boutaud *1, Marie Michael *1, Céline Banal 2, Damelys Calderon 1, Sarah Farcy 1, Julie Pernelle 1, Nicolas Goudin 3, Camille Maillard 1, Clémantine Dimartino 1, Cécile Deleschaux 1, Sébastien Dupichaud 4, Corinne Lebreton 1, Sophie Saunier 1, Tania Attié-Bitach 1,5, Nadia Bahi-Buisson 1,6, Nathalie Lefort 2, Sophie Thomas 1
1Imagine Institute, INSERM UMR 1163, Université de Paris, 2Imagine Institute, iPSC Core Facility, INSERM UMR U1163, Université de Paris, 3Necker Bio-image Analysis platform of the SFR Necker, 4Imagine Institute, Cell Imaging Platform, INSERM-US24-CNRS UMS 3633 Structure Fédérative de Recherche Necker, INSERM UMR U1163, Université de Paris, 5Fédération de Génétique, Hôpital Necker-Enfants Malades, Assistance Publique Hôpitaux de Paris, 6Pediatric Neurology, APHP- Necker Enfants Malades Hospital
We present detailed protocols for the generation and characterization of 2D and 3D human induced pluripotent stem cell (hIPSC)-based models of neocortical development as well as complementary methodologies enabling qualitative and quantitative analysis of primary cilium (PC) biogenesis and function.
