S'identifier

NYU Grossman School of Medicine

2 ARTICLES PUBLISHED IN JoVE

Cancer Research

A Robust Discovery Platform for the Identification of Novel Mediators of Melanoma Metastasis

Arman Alberto Sorin Shadaloey^1,2, Alcida Karz^1,2, Rana S. Moubarak^1,2, Praveen Agrawal^1,2, Grace Levinson^1,2, Kevin Kleffman^1,2, Orlando Aristizabal^3,4, Iman Osman^2,5, Youssef Z. Wadghiri^3,4, Eva Hernando^1,2

¹Department of Pathology, NYU Grossman School of Medicine, ²Interdisciplinary Melanoma Cooperative Group, Perlmutter Cancer Center, ³Department of Radiology, NYU Grossman School of Medicine, ⁴Preclinical Imaging Core, NYU Grossman School of Medicine, ⁵Ronald Perelman Department of Dermatology, NYU Grossman School of Medicine

This article describes a workflow of techniques employed for testing novel candidate mediators of melanoma metastasis and their mechanism(s) of action.

Biology

Visualizing Single-Stranded DNA Foci in the G1 Phase of the Cell Cycle

Qingyue Zhang^1,2, Marc A. Kerzhnerman^1,2, Nelson García-Vázquez^1,2,3, Gergely Rona^1,2,4,5

¹Department of Biochemistry and Molecular Pharmacology, NYU Grossman School of Medicine, ²The Laura and Isaac Perlmutter Cancer Center, NYU Langone Health, ³Department of Cell Biology, NYU Grossman School of Medicine, ⁴Howard Hughes Medical Institute, NYU Grossman School of Medicine, ⁵Institute of Enzymology, Centre of Excellence of the Hungarian Academy of Sciences, HUN-REN Research Centre for Natural Sciences

The following protocol presents the detection of single-stranded DNA foci in the G1 phase of the cell cycle utilizing cell cycle synchronization followed by RPA2 immunofluorescent staining.