Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron MicroscopeAdrian Koh *1, Sagar Khavnekar *2, Wen Yang 1, Dimple Karia 1, Dennis Cats 1, Rob van der Ploeg 1, Fanis Grollios 1, Oliver Raschdorf 1, Abhay Kotecha 1, Daniel Němeček 3
1Materials and Structural Analysis Division, Thermo Fisher Scientific, 2Max Planck Institute of Biochemistry, Molecular Structural Biology, 3Materials and Structural Analysis Division, Thermo Fisher Scientific
High-resolution cryo-EM maps of macromolecules can be also achieved by using 200 kV TEM microscopes. This protocol shows the best practices for setting accurate optics alignments, data acquisition schemes, and selection of imaging areas that are all essential for the successful collection of high-resolution datasets using a 200 kV TEM.
