Isolation and Analysis of Traceable and Functionalized Extracellular Vesicles from the Plasma and Solid TissuesYuan Cao *1,2, Ji-Yu Qiu *1,3, Da Chen 1,2, Chen-Yao Li 1,4, Shu-Juan Xing 1,5, Chen-Xi Zheng 1, Xin Liu 2, Yan Jin 1,6, Bing-Dong Sui 1
1State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi International Joint Research Center for Oral Diseases, Center for Tissue Engineering, School of Stomatology, The Fourth Military Medical University, 2Department of Orthodontics, School of Stomatology, The Fourth Military Medical University, 3Department of VIP Dental Care, School of Stomatology, The Fourth Military Medical University, 4School of Basic Medicine, The Fourth Military Medical University, 5College of Life Science, Northwest University, 6Xi'an Institute of Tissue Engineering and Regenerative Medicine
The present protocol describes a method to extract extracellular vesicles from the peripheral blood and solid tissues with subsequent profiling of surface antigens and protein cargos.