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Xi'an Institute of Tissue Engineering and Regenerative Medicine

2 ARTICLES PUBLISHED IN JoVE

Biology

Isolation and Analysis of Traceable and Functionalized Extracellular Vesicles from the Plasma and Solid Tissues

Yuan Cao^*1,2, Ji-Yu Qiu^*1,3, Da Chen^1,2, Chen-Yao Li^1,4, Shu-Juan Xing^1,5, Chen-Xi Zheng¹, Xin Liu², Yan Jin^1,6, Bing-Dong Sui¹

¹State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi International Joint Research Center for Oral Diseases, Center for Tissue Engineering, School of Stomatology, The Fourth Military Medical University, ²Department of Orthodontics, School of Stomatology, The Fourth Military Medical University, ³Department of VIP Dental Care, School of Stomatology, The Fourth Military Medical University, ⁴School of Basic Medicine, The Fourth Military Medical University, ⁵College of Life Science, Northwest University, ⁶Xi'an Institute of Tissue Engineering and Regenerative Medicine

The present protocol describes a method to extract extracellular vesicles from the peripheral blood and solid tissues with subsequent profiling of surface antigens and protein cargos.

Biology

Isolation, Characterization, and Therapeutic Application of Extracellular Vesicles from Cultured Human Mesenchymal Stem Cells

Shu-Juan Xing^1,2, Kai-Chao Zhang^2,3, Si-Yuan Tang^2,4, Lu Liu^2,5, Yuan Cao^2,5, Chen-Xi Zheng², Bing-Dong Sui², Yan Jin^2,3

¹College of Life Science, Northwest University, ²State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi International Joint Research Center for Oral Diseases, Center for Tissue Engineering, School of Stomatology, Fourth Military Medical University, ³Xi'an Institute of Tissue Engineering and Regenerative Medicine, ⁴School of Basic Medicine, Fourth Military Medical University, ⁵Department of Orthodontics, School of Stomatology, Fourth Military Medical University

The present protocol describes the differential centrifugation for isolating and characterizing representative EVs (exosomes and microvesicles) from cultured human MSCs. Further applications of these EVs are also explained in this article.