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Developmental Biology

Live Imaging of Early Cardiac Progenitors in the Mouse Embryo
Miquel Sendra 1, Jorge Mañes 1, Jorge N. Domínguez 2,3, Miguel Torres 1
1Cardiovascular Regeneration Program, Centro Nacional de Investigaciones Cardiovasculares (CNIC), 2Cardiovascular Development Group, Department of Experimental Biology, University of Jaén, Jaén, 3Fundación Medina, Granada

We present a detailed protocol for mouse embryo culture and imaging that enables 3D + time imaging of cardiac progenitor cells. This video-toolkit addresses the key skills required for successful live imaging otherwise hard to acquire from text-only publications.

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