Microscope Preparation, Embryo Mounting, and Image Acquisition
5:56
Results: 3D Live Imaging of Cardiac Progenitor Cells in the Mouse Embryo
6:35
Conclusion
Transcription
This protocol allows imaging mouse embryos as they develop. This enables studying early cardiogenesis in detail. Like taking still images of fake embryos, live imaging gives full access to study dynamic process in embryo development.
The ski
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We present a detailed protocol for mouse embryo culture and imaging that enables 3D + time imaging of cardiac progenitor cells. This video-toolkit addresses the key skills required for successful live imaging otherwise hard to acquire from text-only publications.