A High-Throughput Platform for Culture and 3D Imaging of OrganoidsGianluca Grenci 1,2, Florian Dilasser 1, Saburnisha Binte Mohamad Raffi 1, Marion Marchand 1, Mona Suryana 1, Geetika Sahni 1, Virgile Viasnoff 1,3,4, Anne Beghin 1,5
1Mechanobiology Institute (MBI), National University of Singapore, 2Biomedical Engineering Department, National University of Singapore, 3Department of Biological Sciences, National University of Singapore, 4IRL 3639 CNRS, 5Department of Microbiology & Immunology, Immunology Translational Research Program, Yong Loo Lin School of Medicine, National University of Singapore
This paper presents a fabrication protocol for a new type of culture substrate with hundreds of microcontainers per mm2, in which organoids can be cultured and observed using high-resolution microscopy. The cell seeding and immunostaining protocols are also detailed.
