Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiaeGisela Cairo *1, Anne MacKenzie *1, Dai Tsuchiya 1,2, Soni Lacefield 1
1Department of Biology, Indiana University, 2Stowers Institute for Biomedical Research
Time-lapse microscopy is a valuable tool for studying meiosis in budding yeast. This protocol describes a method that combines cell-cycle synchronization, time-lapse microscopy, and conditional depletion of a target protein to demonstrate how to study the function of a specific protein during meiotic chromosome segregation.
