murine pain behavioral testing to study the effect of tuina therapy

Protocole Tuina normalisé pour le modèle de compression Rat DRG

In clinical settings, it is common to observe neurological pathological pain caused by nerve root compression due to various reasons. The most typical form of this neuropathic pain is lumbar disc herniation (LDH), which is often persistent, recurrent, and difficult to cure. Approximately 9% of the global population is affected by LDH, leading to significant social and economic burdens1. The incidence of this type of neuropathic pain is increasing yearly, with a trend toward younger patients, due to changes in human production and lifestyle2. Despite the use of non-steroidal painkillers, patients&#39; symptoms cannot be completely alleviated. As a result, alternative therapies, such as Tuina, for treating pain caused by LDH have gained increasing attention.
Tuina therapy, a form of conservative treatment for LDH, is widely recommended in various clinical practice guidelines worldwide for preventing and treating lower back pain3,4. Research has shown that Tuina can significantly lower inflammatory factors such as serum IL-6 and tumor necrosis factor-alpha (TNF-&#945;) levels in LDH patients while improving patients&#39; pain and lumbar function impairment5. However, the specific mechanism behind Tuina therapy&#39;s pain-relieving effects remains unclear.
Animal models are a valuable tool for studying neuropathic pain caused by LDH6. They allow for behavioral measurements to evaluate the effectiveness of Tuina therapy and provide samples of the pathological physiology of LDH. For example, samples from the dorsal root ganglia in the thigh can be taken to verify changes in dorsal root ganglion cells. The chronic compression of the Dorsal Root Ganglion (CCD) model is commonly used to evaluate the pathological physiology of LDH, as it causes damage to the morphology of dorsal root ganglion cells that are consistent with the pathological changes seen in clinical cases of nerve compression caused by disc herniation7.
Many scholars have conducted several animal experiments on acupressure analgesia8,9,10. However, when implementing acupressure operations on animal models, they often imitate human acupressure. The therapeutic effect of acupressure is affected by factors such as the size, frequency, and the direction of the applied force11,12,13. If the experiment lacks a unified acupressure standard, such as the force, frequency, and duration of the operation, this may cause some deviation in the experimental results. This article introduces a set of acupressure treatment plans based on the characteristics of CCD rats, and promotes the development of standardized acupressure operations in animal models.

Pleins feux sur l’auteur : Effet analgésique de la tuina sur des modèles de rats avec compression de la douleur ganglionnaire de la racine dorsale  

Effet analgésique du Tuina sur les modèles de rats avec compression de la douleur ganglionnaire de la racine dorsale

Watch this Scientific Journal Video about Murine Pain Behavioral Testing to Study the Effect of Tuina Therapy at JoVE.com

Test comportemental de la douleur murine pour étudier l’effet de la thérapie Tuina

Pour effectuer le test de Von Cray, commencez par placer les rats dans un compartiment en verre trempé transparent sur un support de grille métallique à une hauteur de 40 centimètres. Ensuite, mesurez le seuil de retrait mécanique avec les fibres électroniques Von Frey. Stimulez la patte du rat jusqu’à ce qu’elle réagisse sensiblement, par exemple en soulevant la patte ou en l’évitant.Pour évaluer la latence de retrait de la patte, ou PWL à l’aide de la méthode Hargreaves, concentrez le projecteur sur le centre de la patte du rat et appuyez sur le bouton de démarrage. Surveillez les comportements du rat tels que la rétraction des pieds ou le léchage des pattes, et appuyez à nouveau sur le bouton de démarrage pour enregistrer l’heure. Effectuez le test sur le même membre postérieur trois fois de suite.Après 17 jours de traitement par Tuina, une différence significative a été observée dans les seuils de retrait des pattes entre les rats CCD recevant un traitement par Tuina et le groupe CCD non traité. Les rats du groupe CCD recevant une thérapie Tuina ont montré une amélioration du seuil de douleur dès le début du traitement. Les seuils de douleur thermique différaient significativement entre le groupe CCD et le groupe Tuina à partir du 14e jour.

murine-pain-behavioral-testing-to-study-the-effect-of-tuina-therapy

Research

JoVE Journal

Medicine

Murine Pain Behavioral Testing to Study the Effect of Tuina Therapy

120 vues. Pour effectuer le test de Von Cray, commencez par placer les rats dans un compartiment en verre trempé transparent sur un support de grille métallique à une hauteur de 40 centimètres. Ensuite, mesurez le seuil de retrait mécanique avec les fibres électroniques Von Frey. Stimulez la patte du rat jusqu’à ce qu’elle réagisse sensiblement, par exemple en soulevant la patte ou en l’évitant.Pour évaluer la latence de retrait de la patte, ou PWL à l’aide de la méthode Harg...

Vidéo: Test comportemental de la douleur murine pour étudier l’effet de la thérapie Tuina

Analgesic Effect of Tuina on Rat Models with Compression of the Dorsal Root Ganglion Pain

Neuropathic pain is a prevalent condition that affects 6.9%-10% of the population and results from nerve damage due to various etiologies, such as lumbar disc herniation, spinal canal stenosis, and intervertebral foramen stenosis. Although Tuina, a traditional Chinese manual therapy, has shown analgesic effects in clinical practice for the treatment of neuropathic pain, its underlying neurobiological mechanisms remain unclear. Animal models are essential for elucidating the basic principles of Tuina. In this study, we propose a standardized Tuina protocol for rats with compression of the dorsal root ganglion (DRG), which involves inducing DRG compression by inserting a stainless steel rod into the intervertebral foramen, performing Tuina manipulation with specific parameters of location, intensity, and frequency in a controlled environment, and assessing the behavioral and histopathological outcomes of Tuina treatment. This article also discusses the potential clinical implications and limitations of the study and suggests directions for future research on Tuina.

This article presents a manipulation for treating chronic compression of the dorsal root ganglion in rats using Tuina therapy, along with a method for evaluating its effectiveness based on pain behavior and histopathological results.

Neuropathic pain is a prevalent condition that affects 6.9%-10% of the population and results from nerve damage due to various etiologies, such as lumbar ...